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CC BY 4.0 · Eur J Dent 2024; 18(04): 1149-1156
DOI: 10.1055/s-0044-1782189
Original Article

The Potential of QP3VH-Chitosan Peptide as Biomimetic Remineralization in Early Dental Caries Treatment: An In Vitro Study

Prima Agusmawanti 
1   Doctoral Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
2   Departement of Pediatric Dentistry, Faculty of Dentistry, Universitas Islam Sultan Agung, Indonesia
,
Diatri Nari Ratih 
3   Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
,
Nunuk Purwanti 
4   Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
,
Tri Joko Raharjo 
5   Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia
› Author Affiliations Funding The author would like to thank to Universitas Gadjah Mada for the financial support through program of Rekognisi Tugas Akhir (RTA) number: 5075/UN1.P.II/Dit-Lit/PT.01.01/2023.
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Abstract

Objectives The development of remineralization biomimetics using organic peptide molecules is expected to resemble the hydroxyapatite (HA) mineralization process in tooth enamel. The development of an amelogenin derivative peptide combined with antimicrobial peptide was designed, resulting in QP3VH. This combination then was mixed with chitosan as a carrier. This study aimed to evaluate the biomimetic efficacy of QP3VH as a remineralizing agent combined with chitosan.

Materials and Methods Fifty deciduous mandibular incisor enamel samples were used in this study. The artificial enamel lesions were created on a buccal surface and were randomly assigned to five groups of 10 each according to the remineralizing agent used: QP3VH, NaF, QP3VH + NaF, QP3VH + CS (QP3VH + chitosan), and saline distilled water (SDW). Each group was performed pH cycling for seven days. Enamel surface morphology and evaluation of mineral content Ca/P (calcium and phosphate) using scanning electron microscopy and energy dispersive X-ray analysis. The assessment was carried out, after demineralization, and after application with remineralization agents.

Statistical Analysis Data were analyzed using a one-way analysis of variance followed by least significance difference post-hoc test. The paired t-test was utilized to compare the demineralization and remineralization results. The significance level used was 95%.

Results The remineralized group exhibited a significant increase in calcium and phosphate content on the enamel surface (p <0.05), and QP3VH + CS produced the maximum Ca/P mass percent after remineralization.

Conclusion Combining QP3VH with chitosan produces greatest remineralization than QP3VH, QP3VH + NaF, Naf, and SDW; therefore, QP3VH peptide has potential as a remineralizing agent, in the future

Keywords

biomimetic - remineralization - caries - QP3VH peptide - chitosan - NaF


Publication History

Article published online:
17 May 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Petersen PE, Bourgeois D, Ogawa H, Estupinan-Day S, Ndiaye C. The global burden of oral diseases and risks to oral health. Bull World Health Organ 2005; 83 (09) 661-669
    PubMedSearch in Google Scholar
  • 2 Manton DJ. Child dental caries - a global problem of inequality. EClinicalMedicine 2018; 1 (01) 3-4
    CrossrefPubMedSearch in Google Scholar
  • 3 Durhan MA, Ozsalih S, Gokkaya B, Kulan PY, Kargul B. Caries preventive effects of theobromine containing toothpaste on early childhood caries: preliminary results. Acta Stomatol Croat 2021; 55 (01) 18-27
    CrossrefPubMedSearch in Google Scholar
  • 4 Tulumbaci F, Gungormus M. In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish. J Appl Oral Sci 2020; 28: e20200259
    CrossrefPubMedSearch in Google Scholar
  • 5 Yang Y, Lv XP, Shi W. et al. 8DSS-promoted remineralization of initial enamel caries in vitro. J Dent Res 2014; 93 (05) 520-524
    CrossrefPubMedSearch in Google Scholar
  • 6 Chen L, Yuan H, Tang B, Liang K, Li J. Biomimetic remineralization of human enamel in the presence of polyamidoamine dendrimers in vitro. Caries Res 2015; 49 (03) 282-290
    CrossrefPubMedSearch in Google Scholar
  • 7 Shihabi S, AlNesser S, Comisi JC. Comparative remineralization efficacy of topical Novamin and fluoride on incipient enamel lesions in primary teeth: scanning electron microscope and Vickers microhardness evaluation. Eur J Dent 2021; 15 (03) 420-424
    Thieme ConnectPubMedSearch in Google Scholar
  • 8 Schmidlin P, Zobrist K, Attin T, Wegehaupt F. In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides. J Appl Oral Sci 2016; 24 (01) 31-36
    CrossrefPubMedSearch in Google Scholar
  • 9 Amaechi BT, AbdulAzees PA, Alshareif DO. et al. Comparative efficacy of a hydroxyapatite and a fluoride toothpaste for prevention and remineralization of dental caries in children. BDJ Open 2019; 5: 18
    CrossrefPubMedSearch in Google Scholar
  • 10 Ding L, Han S, Wang K. et al. Remineralization of enamel caries by an amelogenin-derived peptide and fluoride in vitro . Regen Biomater 2020; 7 (03) 283-292
    CrossrefPubMedSearch in Google Scholar
  • 11 Chu J, Feng X, Guo H, Zhang T, Zhao H, Zhang Q. Remineralization efficacy of an amelogenin-based synthetic peptide on carious lesions. Front Physiol 2018; 9: 842
    CrossrefPubMedSearch in Google Scholar
  • 12 Wang Y, Hu D, Cui J. et al. Unraveling the mechanism for an amelogenin-derived peptide regulated hydroxyapatite mineralization via specific functional domain identification. J Mater Chem B Mater Biol Med 2020; 8 (45) 10373-10383
    CrossrefPubMedSearch in Google Scholar
  • 13 Wang X, Wang Y, Wang K. et al. Bifunctional anticaries peptides with antibacterial and remineralizing effects. Oral Dis 2019; 25 (02) 488-496
    CrossrefPubMedSearch in Google Scholar
  • 14 Ren Q, Ding L, Li Z. et al. Chitosan hydrogel containing amelogenin-derived peptide: Inhibition of cariogenic bacteria and promotion of remineralization of initial caries lesions. Arch Oral Biol 2019; 100: 42-48
    CrossrefPubMedSearch in Google Scholar
  • 15 Ruan Q, Zhang Y, Yang X, Nutt S, Moradian-Oldak J. An amelogenin-chitosan matrix promotes assembly of an enamel-like layer with a dense interface. Acta Biomater 2013; 9 (07) 7289-7297
    CrossrefPubMedSearch in Google Scholar
  • 16 Xiao Z, Que K, Wang H. et al. Rapid biomimetic remineralization of the demineralized enamel surface using nano-particles of amorphous calcium phosphate guided by chimaeric peptides. Dent Mater 2017; 33 (11) 1217-1228
    CrossrefPubMedSearch in Google Scholar
  • 17 Argenta RMO, Tabchoury CPM, Cury JA. A modified pH-cycling model to evaluate fluoride effect on enamel demineralization. Pesqui Odontol Bras 2003; 17 (03) 241-246
    CrossrefPubMedSearch in Google Scholar
  • 18 Lv X, Yang Y, Han S. et al. Potential of an amelogenin based peptide in promoting remineralization of initial enamel caries. Arch Oral Biol 2015; 60 (10) 1482-1487
    CrossrefPubMedSearch in Google Scholar
  • 19 Zhang OL, Niu JY, Yu OY, Mei ML, Jakubovics NS, Chu CH. Peptide designs for use in caries management: a systematic review. Int J Mol Sci 2023; 24 (04) 4247
    CrossrefPubMedSearch in Google Scholar
  • 20 Thimmaiah C, Shetty P, Shetty SB, Natarajan S, Thomas NA. Comparative analysis of the remineralization potential of CPP-ACP with fluoride, tri-calcium phosphate and nano hydroxyapatite using SEM/EDX - an in vitro study. J Clin Exp Dent 2019; 11 (12) e1120-e1126
    PubMedSearch in Google Scholar
  • 21 Fan Y, Sun Z, Moradian-Oldak J. Controlled remineralization of enamel in the presence of amelogenin and fluoride. Biomaterials 2009; 30 (04) 478-483
    CrossrefPubMedSearch in Google Scholar
  • 22 Palmer LC, Newcomb CJ, Kaltz SR, Spoerke ED, Stupp SI. Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel. Chem Rev 2008; 108 (11) 4754-4783
    CrossrefPubMedSearch in Google Scholar
  • 23 Kirkham J, Firth A, Vernals D. et al. Self-assembling peptide scaffolds promote enamel remineralization. J Dent Res 2007; 86 (05) 426-430
    CrossrefPubMedSearch in Google Scholar
  • 24 Xiang C, Ran J, Yang Q, Li W, Zhou X, Zhang L. Effects of enamel matrix derivative on remineralisation of initial enamel carious lesions in vitro. Arch Oral Biol 2013; 58 (04) 362-369
    CrossrefPubMedSearch in Google Scholar
  • 25 Hosseini S, Naderi-Manesh H, Mountassif D, Cerruti M, Vali H, Faghihi S. C-terminal amidation of an osteocalcin-derived peptide promotes hydroxyapatite crystallization. J Biol Chem 2013; 288 (11) 7885-7893
    CrossrefPubMedSearch in Google Scholar
  • 26 Kamal D, Hassanein H, Elkassas D, Hamza H. Complementary remineralizing effect of self-assembling peptide (P11-4) with CPP-ACPF or fluoride: an in vitro study. J Clin Exp Dent 2020; 12 (02) e161-e168
    CrossrefPubMedSearch in Google Scholar
  • 27 Ren Q, Li Z, Ding L. et al. Anti-biofilm and remineralization effects of chitosan hydrogel containing amelogenin-derived peptide on initial caries lesions. Regen Biomater 2018; 5 (02) 69-76
    CrossrefPubMedSearch in Google Scholar
  • 28 Arnaud TM, de Barros Neto B, Diniz FB. Chitosan effect on dental enamel de-remineralization: an in vitro evaluation. J Dent 2010; 38 (11) 848-852
    CrossrefPubMedSearch in Google Scholar