Download PDF
CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0044-1782211
Original Article

The Potential Role of Reactive Oxygen Species Produced by Low-Density Neutrophils in Periodontitis

Ali Omran Mousa 
1   Department of Periodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq
,
Ali Hussien Abass Al Hussaini 
2   Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Baghdad, Baghdad, Iraq
,
Hashim Mueen Hussein 
3   Department of Conservative Dentistry, College of Dentistry, Mustansiriyah University, Baghdad, Iraq
› Author Affiliations
› Further Information
Also available at
   Permissions and Reprints

Abstract

Objective Neutrophils own an arsenal of dischargeable chemicals that enable them to handle bacterial challenges, manipulating innate immune response and actual participation in acquired immunity. The reactive oxygen species (ROS) are one of the most important chemicals that neutrophils discharge to eradicate pathogens. Despite their beneficial role, the ROS were strongly correlated to periodontal tissue destruction. Lowdensity neutrophils (LDN) have been recognized for producing enhanced quantities of ROS. However, the potential role of ROS produced by LDN in periodontitis is unknown. The aim of the study was to investigate the impact of ROS produced by LDN in periodontal diseases.

Materials and Methods Venous blood and periodontal parameters were obtained from 100 systemically healthy subjects divided into 40 participants with healthy periodontium in the control group and 60 with unstable periodontitis in the study group. Flow cytometry was used to measure the production of ROS by LDN in both groups.

Statistical Analysis The data were analyzed for normal distribution using the Shapiro-Wilk test at p < 0.05, Spearman's correlations, and Mann-Whitney U test. Statistical analysis was performed in SPSS v25.

Results No difference between the groups had been obtained in ROS production by LDN. However, a significant positive correlation existed between ROS and clinical attachment loss in periodontitis.

Conclusion LDN exhibits the same ROS generation capacity in the control and periodontitis groups.

Keywords

periodontitis - reactive oxygen species - low-density neutrophils


Publication History

Article published online:
14 May 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 

  • References

  • 1 Saliem SS, Bede SY, Cooper PR, Abdulkareem AA, Milward MR, Abdullah BH. Pathogenesis of periodontitis: a potential role for epithelial-mesenchymal transition. Jpn Dent Sci Rev 2022; 58: 268-278
    CrossrefPubMedGoogle Scholar
  • 2 Mahmood AA, Abbas RF. Assessment of NLRP3 gene polymorphisms with periodontitis as compared with healthy periodontium in Iraqi Arabs patients. Eur J Dent 2023; 17 (04) 1338-1348
    Article in Thieme ConnectPubMedGoogle Scholar
  • 3 Hussein HM, Mahmood AA, Alberaqdar FA. The prevalence and relationship of root caries depth and gingival recession among different Iraqi groups. Mustansiria Dent J 2015; 12 (01) 144-155
    Google Scholar
  • 4 Saeed NA, Hussein HM, Mahmood AA. Prevalence of dental anxiety in relation to sociodemographic factors using two psychometric scales in Baghdad. Mustansiria Dent J 2017; 14 (01) 38-50
    CrossrefGoogle Scholar
  • 5 Mahmood HK, Al-Ghurabi BH. Low frequency of active HCMV infection among chronic periodontitis patients. Biochem Cell Arch 2020; 20 (01) 847-851
    Google Scholar
  • 6 Mulawarmanti D, Parisihni K. eds. The effect of Sticopus hermanii-hyperbaric oxygen therapy to inflammatory response of diabetic periodontitis. In: IOP Conference Series: Earth and Environmental Science. Bristol, UK: IOP Publishing; 2019
    CrossrefGoogle Scholar
  • 7 Mousa AO, Saliem SS, Abdullah BH, Raad Abdulbaqi H. Age gender and site effect on immunohistochemical expression of TGF-β1 and IFN-γ in hereditary gingival fibromatosis. J Glob Pharma Technol 2019; 11 (02) 542-547
    Google Scholar
  • 8 McClean C, Harris RA, Brown M, Brown JC, Davison GW. Effects of exercise intensity on postexercise endothelial function and oxidative stress. Oxid Med Cell Longev 2015; 2015: 723679
    CrossrefPubMedGoogle Scholar
  • 9 Di Meo S, Reed TT, Venditti P, Victor VM. Role of ROS and RNS sources in physiological and pathological conditions. Oxid Med Cell Longev 2016; 2016: 1245049
    CrossrefPubMedGoogle Scholar
  • 10 Blanco-Camarillo C, Alemán OR, Rosales C. Low-density neutrophils in healthy individuals display a mature primed phenotype. Front Immunol 2021; 12: 672520
    CrossrefPubMedGoogle Scholar
  • 11 Budhy TI, Arundina I, Surboyo MDC, Halimah AN. The effects of rice husk liquid smoke in Porphyromonas gingivalis-induced periodontitis. Eur J Dent 2021; 15 (04) 653-659
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Abdulhameed VS, Saliem SS, Hassan TA. Evaluation of crestal bone loss and alkaline phosphatase level in saliva according to different flap designs in single-tooth dental implant surgery (a clinical comparative study). Biomed Pharmacol J 2017; 10 (04) 1863-1869
    CrossrefGoogle Scholar
  • 13 Baser U, Gamsiz-Isik H, Cifcibasi E, Ademoglu E, Yalcin F. Plasma and salivary total antioxidant capacity in healthy controls compared with aggressive and chronic periodontitis patients. Saudi Med J 2015; 36 (07) 856-861
    CrossrefPubMedGoogle Scholar
  • 14 Chapple ILC, Mealey BL, Van Dyke TE. et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol 2018; 89 (Suppl. 01) S74-S84
    CrossrefPubMedGoogle Scholar
  • 15 Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol 2018; 89 (Suppl. 01) S159-S172
    CrossrefPubMedGoogle Scholar
  • 16 Silness J, Löe H. Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 1964; 22 (01) 121-135
    CrossrefPubMedGoogle Scholar
  • 17 Fine N, Tasevski N, McCulloch CA, Tenenbaum HC, Glogauer M. The neutrophil: constant defender and first responder. Front Immunol 2020; 11: 571085
    CrossrefPubMedGoogle Scholar
  • 18 Nauseef WM. Neutrophils, from cradle to grave and beyond. Immunol Rev 2016; 273 (01) 5-10
    CrossrefPubMedGoogle Scholar
  • 19 Lacy P, Eitzen G. Control of granule exocytosis in neutrophils. Front Biosci 2008; 13 (01) 5559-5570
    CrossrefPubMedGoogle Scholar
  • 20 Zeng MY, Miralda I, Armstrong CL, Uriarte SM, Bagaitkar J. The roles of NADPH oxidase in modulating neutrophil effector responses. Mol Oral Microbiol 2019; 34 (02) 27-38
    CrossrefPubMedGoogle Scholar
  • 21 Papayannopoulos V. Neutrophil extracellular traps in immunity and disease. Nat Rev Immunol 2018; 18 (02) 134-147
    CrossrefPubMedGoogle Scholar
  • 22 Leliefeld PH, Wessels CM, Leenen LP, Koenderman L, Pillay J. The role of neutrophils in immune dysfunction during severe inflammation. Crit Care 2016; 20: 73
    CrossrefPubMedGoogle Scholar
  • 23 Nauseef WM, Borregaard N. Neutrophils at work. Nat Immunol 2014; 15 (07) 602-611
    CrossrefPubMedGoogle Scholar
  • 24 Scapini P, Marini O, Tecchio C, Cassatella MA. Human neutrophils in the saga of cellular heterogeneity: insights and open questions. Immunol Rev 2016; 273 (01) 48-60
    CrossrefPubMedGoogle Scholar
  • 25 Ohki T, Itabashi Y, Kohno T. et al. Detection of periodontal bacteria in thrombi of patients with acute myocardial infarction by polymerase chain reaction. Am Heart J 2012; 163 (02) 164-167
    CrossrefPubMedGoogle Scholar
  • 26 Abdulkareem AA, Al-Taweel FB, Al-Sharqi AJB, Gul SS, Sha A, Chapple ILC. Current concepts in the pathogenesis of periodontitis: from symbiosis to dysbiosis. J Oral Microbiol 2023; 15 (01) 2197779
    CrossrefPubMedGoogle Scholar
  • 27 Kanzaki H, Shinohara F, Kajiya M, Kodama T. The Keap1/Nrf2 protein axis plays a role in osteoclast differentiation by regulating intracellular reactive oxygen species signaling. J Biol Chem 2013; 288 (32) 23009-23020
    CrossrefPubMedGoogle Scholar
  • 28 Chapple ILC, Brock G, Eftimiadi C, Matthews JB. Glutathione in gingival crevicular fluid and its relation to local antioxidant capacity in periodontal health and disease. Mol Pathol 2002; 55 (06) 367-373
    CrossrefPubMedGoogle Scholar
  • 29 Patil VS, Patil VP, Gokhale N, Acharya A, Kangokar P. Chronic periodontitis in type 2 diabetes mellitus: oxidative stress as a common factor in periodontal tissue injury. J Clin Diagn Res 2016; 10 (04) BC12-BC16
    PubMedGoogle Scholar
  • 30 Liu C, Mo L, Niu Y, Li X, Zhou X, Xu X. The role of reactive oxygen species and autophagy in periodontitis and their potential linkage. Front Physiol 2017; 8: 439
    CrossrefPubMedGoogle Scholar
  • 31 Daley JM, Thomay AA, Connolly MD, Reichner JS, Albina JE. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol 2008; 83 (01) 64-70
    CrossrefPubMedGoogle Scholar
  • 32 Damuzzo V, Pinton L, Desantis G. et al. Complexity and challenges in defining myeloid-derived suppressor cells. Cytometry B Clin Cytom 2015; 88 (02) 77-91
    CrossrefPubMedGoogle Scholar
  • 33 Rosales C. Neutrophil: a cell with many roles in inflammation or several cell types?. Front Physiol 2018; 9: 113
    CrossrefPubMedGoogle Scholar
  • 34 Dumitru CA, Moses K, Trellakis S, Lang S, Brandau S. Neutrophils and granulocytic myeloid-derived suppressor cells: immunophenotyping, cell biology and clinical relevance in human oncology. Cancer Immunol Immunother 2012; 61 (08) 1155-1167
    CrossrefPubMedGoogle Scholar