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CC BY 4.0 · Eur J Dent 2024; 18(04): 1135-1141
DOI: 10.1055/s-0044-1782212
Original Article

Potency of the Combination of Chitosan and Hydroxyapatite on Angiogenesis and Fibroblast Cell Proliferation in Direct Pulp Capping of Rattus norvegicus

Sularsih Sularsih 
1   Department of Dental Materials, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Wanli Fransiska
2   Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Syifa Salsabila
2   Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Fitria Rahmitasari
1   Department of Dental Materials, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Diana Soesilo
3   Department of Conservative Dentistry, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Widyasri Prananingrum
1   Department of Dental Materials, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
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Abstract

Objectives The aim of this research was to analyze expression of vascular endothelial growth factor (VEGF), blood vessels, and fibroblast cell proliferation in direct pulp capping treatment of Rattus norvegicus using a combination of chitosan and hydroxyapatite paste.

Materials and Methods The samples were male R. norvegicus strains of Wistar rats, weighing 200 to 250 g and aged between 8 and 16 weeks. The occlusal surface of the molars of R. norvegicus was prepared with class I cavity and then perforated with the tip of an explorer. Sixty male R. Norvegicus rats were divided into the following: control group (KA) in which the cavity was filled with glass ionomer cement; control group (KB), in which the cavity was filled with Ca(OH)2; PA group, in which the cavity was filled with chitosan (CH); PB group, in which the cavity was filled with hydroxyapatite (HA); and PC group, in which the cavity was filled with chitosan and hydroxyapatite (CH-HA). Each group was divided into 3-, 7-, and 14-day observation groups. The mandibular bone of the molar was cut and histopathological examination was performed to analyze the blood vessels and fibroblast cell proliferation. Immunohistochemistry examination was done to examine the expression of VEGF.

Statistical Analysis The data variation was analyzed with One Way Analysis of Variance (ANOVA) test and continued with multiple comparison Least Significant (LSD) test to determine the different pairs of group.

Results Analysis of variance (ANOVA) showed a significant increase in the expression of VEGF, blood vessels, and fibroblast cell proliferations (p ≤ 0.05;), especially in the PC group compared to the other four groups. The least significant test (LSD) test showed significant differences between the groups on the expression of VEGF, blood vessels, and fibroblast cell proliferations.

Conclusion The combination of chitosan and hydroxyapatite could promote healing of direct pulp capping treatment by increasing the expression of VEGF, blood vessel, and fibroblast cell proliferation.

Keywords

chitosan - hydroxyapatite - direct pulp capping - VEGF - blood vessel - fibroblast


Publication History

Article published online:
02 May 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Islam R, Islam MRR, Tanaka T, Alam MK, Ahmed HMA, Sano H. Direct pulp capping procedures: evidence and practice. Jpn Dent Sci Rev 2023; 59: 48-61
    CrossrefPubMedGoogle Scholar
  • 2 Whitehouse LL, Thomson NH, Do T, Feichtinger GA. Bioactive molecules for regenerative pulp capping. Eur Cell Mater 2021; 42 (01) 415-437
    CrossrefPubMedGoogle Scholar
  • 3 Si W, Zhou Y, Yu Y. et al. Evaluation of chitosan hydrogel for sustained delivery of VEGF for odontogenic differentiation of dental pulp stem cells. Stem cells. Int J 2019; 1 (01) 1-14
    Google Scholar
  • 4 Mente J, Hufnagel S, Leo M. et al. Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: long-term results. J Endod 2014; 40 (11) 1746-1751
    CrossrefPubMedGoogle Scholar
  • 5 Ravindaran V, Jeevanandan G, Marimuthu M. et al. Comparison of mineral trioxide aggregate and biodentin for open apex management in children with non vital immature permanent teeth: a systematic review. Eur J Dent 2022; 11 (02) 84-93
    Article in Thieme ConnectGoogle Scholar
  • 6 Divband B, Pouya B, Hassanpour M. et al. Towards induction of angiogenesis in dental pulp stem cells using chitosan-based hydrogels releasing basic fibroblast growth factor. BioMed Res Int 2022; 2022: 5401461
    CrossrefPubMedGoogle Scholar
  • 7 Szulc M, Lewandowska K. Biomaterials based on chitosan and its derivatives and their potential in tissue engineering and other biomedical applications: a review. Molecules 2022; 28 (01) 247-264
    CrossrefPubMedGoogle Scholar
  • 8 Gurucharan I, Saravana Karthikeyan B, Mahalaxmi S. et al. Characterization of nano-hydroxyapatite incorporated carboxymethyl chitosan composite on human dental pulp stem cells. Int Endod J 2023; 56 (04) 486-501
    CrossrefPubMedGoogle Scholar
  • 9 Vagropoulou P, Trentsiou M, Georgopoulou A. et al. Hybrid chitosan/gelatin/nanohydroxyapatite scaffolds promote odontogenic differentiation of dental pulp stem cells and in vitro biomineralization. Dent Mater J 2021; 37 (01) e23-e36
    CrossrefPubMedGoogle Scholar
  • 10 Álvarez-Vásquez JL, Castañeda-Alvarado CP. Dental pulp fibroblast: a star cell. J Endod 2022; 48 (08) 1005-1019
    CrossrefPubMedGoogle Scholar
  • 11 Sularsih S. The pore size of chitosan-aloe vera scaffold and its effect on VEGF expressions and woven alveolar bone healing of tooth extraction of Cavia cobaya . Dent J 2020; 53 (03) 115-121
    CrossrefGoogle Scholar
  • 12 Vaca-Cornejo F, Reyes HM, Jiménez SHD, Velázquez RAL, Jiménez JMD. Pilot study using a chitosan-hydroxyapatite implant for guided alveolar bone growth in patients with chronic periodontitis. J Funct Biomater 2017; 8 (03) 29-35
    CrossrefPubMedGoogle Scholar
  • 13 Tanideh N, Dehghani Nazhvani S, Mojtahed Jaberi F. et al. The healing effect of bioglue in articular cartilage defect of femoral condyle in experimental rabbit model. Iran Red Crescent Med J 2011; 13 (09) 629-633
    CrossrefPubMedGoogle Scholar
  • 14 Prananingrum W, Sularsih, Prameswari N. et al. Potential application of chitosan synthesized from Penaeus monodon as direct pulp capping material. Biochem Cell Arch Journal 2021; 21 (02) 3295-3299
    Google Scholar
  • 15 Khurshid Z, Alnaim AJA, Alhashim AAA, Imran E, Adanir N. Future of decellularized dental pulp matrix in regenerative endodontics. Eur J Dent 2022; 16 (04) 737-741
    Article in Thieme ConnectPubMedGoogle Scholar
  • 16 Hargreaves K, Berman L. Cohen's Pathways of the Pulp. 11th ed.. St. Louis, MO: Elsevier; 2016: 85-87
    Google Scholar
  • 17 Shekathar M, Kheur S, Deshpande S. et al. Angiogenic potential of various oral cavity-derived mesenchymal stem cells and cell-derived secretome: a systemic review and meta-analysis. Eur J Dent 2023:10.1055/s-0043-1776315
    Google Scholar
  • 18 Friedlander LT, Coates D, Seymour G, Cullinan M, Rich AM. Vascularity and VEGF/VEGFR2 signaling in the dentine-pulp complex of immature and mature permanent teeth. Eur Endod J 2018; 3 (03) 153-159
    PubMedGoogle Scholar
  • 19 Yalcin M, Arslan U, Dundar A. Evaluation of antibacterial effects of pulp capping agents with direct contact test method. Eur J Dent 2014; 8 (01) 95-99
    Article in Thieme ConnectPubMedGoogle Scholar
  • 20 Soares DG, Basso FG, Scheffel DL, Giro EM, de Souza Costa CA, Hebling J. Biocompatibility of a restorative resin-modified glass ionomer cement applied in very deep cavities prepared in human teeth. Gen Dent 2016; 64 (04) 33-40
    PubMedGoogle Scholar
  • 21 Velickovic Z, Zivkovic D, Bubalo M. et al. The effect of hydroxyapatite and growth factors on reparative dentine formation in the therapy of injured pulp. Vojnosanit Pregl 2021; 78 (02) 147-153
    CrossrefGoogle Scholar
  • 22 Swarup SJ, Rao A, Boaz K, Srikant N, Shenoy R. Pulpal response to nano hydroxyapatite, mineral trioxide aggregate and calcium hydroxide when used as a direct pulp capping agent: an in vivo study. J Clin Pediatr Dent 2014; 38 (03) 201-206
    CrossrefPubMedGoogle Scholar
  • 23 Rahmani F, Larbi Bouamrane O, Ben Bouabdallah A, Atanase LI, Hellal A, Apintiliesei AN. Biomimetic hydroxyapatite crystals growth on phosphorylated chitosan films by in vitro mineralization used as dental substitute materials. Polymers (Basel) 2023; 15 (11) 2470
    CrossrefPubMedGoogle Scholar
  • 24 Putranto AW, Suprastiwi E, Meidyawati R, Agusnar H. Characterization of novel cement-based carboxymethyl chitosan/amorphous calcium phosphate. Eur J Dent 2022; 16 (04) 809-814
    Article in Thieme ConnectPubMedGoogle Scholar
  • 25 S DPA, Solete P, Jeevanandan G. et al. Effect of various irrigant activation methods and its penetration in the apical third of root canal: in vitro study. Eur J Dent 2023; 17 (01) 57-61
    Article in Thieme ConnectPubMedGoogle Scholar
  • 26 Sheela S, Nassar M, AlGhalban FM, Gorduysus MO. In vitro cytotoxicity and mineralization potential of an endodontic bioceramic material. Eur J Dent 2023; 17 (02) 548-555
    Article in Thieme ConnectPubMedGoogle Scholar
  • 27 Bianchi S, Mancini L, Torge D. et al. Bio-morphological reaction of human periodontal ligament fibroblasts to different types of dentinal derivates: in vitro study. Int J Mol Sci 2021; 22 (16) 8681
    CrossrefPubMedGoogle Scholar