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CC BY 4.0 · Eur J Dent 2024; 18(04): 1085-1089
DOI: 10.1055/s-0044-1782215
Original Article

The Influence of Streptococcus mutans Biofilm Formation in a Polymicrobial Environment (Streptococcus gordonii & Porphyromonas gingivalis)

Indah Listiana Kriswandini
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Sidarningsih Sidarningsih
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Adelheid Chrissanda Hermanto
2   Student of Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Pinta Rahayuning Tyas
2   Student of Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Mohammed Ahmed Aljunaid 
3   Department of Dental Medicine, Faculty of Medicine, Taiz University, Taiz, Yemen
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Abstract

Objectives Biofilms play a vital role in the occurrence or worsening of an infectious disease. Streptococcus mutans is a bacterium with the ability to form biofilms that plays a key role in the development of infectious diseases such as dental caries. The formation of biofilms in S. mutans is mediated by quorum sensing. Inhibiting quorum sensing can be considered as one of the approaches to prevent caries. This study aims to investigate the ability of Streptococcus gordonii and Porphyromonas gingivalis bacteria to inhibit the formation of S. mutans biofilm.

Materials and Methods This research was conducted to analyze bacterial biofilm formation and metabolism. The bacteria used are S. mutans (serotype C), S. gordonii (ATCC 5165), and P. gingivalis (ATCC 33277). Biofilm formation was analyzed using the crystal violet assay. Bacterial metabolism was analyzed using the methylthiazol tetrazolium (MTT) assay.

Results The results of the crystal violet assay indicate a decrease in biofilm formation in S. mutans when in the presence of S. gordonii and S. mutans in the presence of P. gingivalis. The results of the MTT assay show no significant change in the bacterial metabolism of S. mutans in the presence of S. gordonii and S. mutans in the presence of P. gingivalis. However, S. mutans with the presence of S. gordonii and P. gingivalis show an increase in biofilm formation and bacterial metabolism.

Conclusion S. gordonii and P. gingivalis are each capable of inhibiting the formation of S. mutans biofilm in a polymicrobial environment.

Keywords

Streptococcus mutans - quorum sensing - infectious disease - biofilm - caries


Publication History

Article published online:
14 May 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Jamal M, Ahmad W, Andleeb S. et al. Bacterial biofilm and associated infections. J Chin Med Assoc 2018; 81 (01) 7-11
    CrossrefPubMedGoogle Scholar
  • 2 Deo PN, Deshmukh R. Oral microbiome: unveiling the fundamentals. J Oral Maxillofac Pathol 2019; 23 (01) 122-128
    CrossrefPubMedGoogle Scholar
  • 3 Lemos JA, Palmer SR, Zeng L. et al. The Biology of Streptococcus mutans. Microbiol Spectr 2019;7(1):10.1128/microbiolspec.GPP3-0051-2018
    Google Scholar
  • 4 Wright PP, Ramachandra SS. Quorum sensing and quorum quenching with a focus on cariogenic and periodontopathic oral biofilms. Microorganisms 2022; 10 (09) 1783
    CrossrefPubMedGoogle Scholar
  • 5 Zayed SM, Aboulwafa MM, Hashem AM, Saleh SE. Biofilm formation by Streptococcus mutans and its inhibition by green tea extracts. AMB Express 2021; 11 (01) 73
    CrossrefPubMedGoogle Scholar
  • 6 Krzyściak W, Jurczak A, Kościelniak D, Bystrowska B, Skalniak A. The virulence of Streptococcus mutans and the ability to form biofilms. Eur J Clin Microbiol Infect Dis 2014; 33 (04) 499-515
    CrossrefPubMedGoogle Scholar
  • 7 Senadheera D, Cvitkovitch DG. Quorum sensing and biofilm formation by Streptococcus mutans. Adv Exp Med Biol 2008; 631: 178-188
    CrossrefPubMedGoogle Scholar
  • 8 Kriswandini IL, Yuna R. Quorum sensing Streptococcus mutans and Lactobacillus salivarius (prospects of prevention of dental caries in the future): narrative review. J Adv Med Sci 2023; 3 (01) 20-22
    Google Scholar
  • 9 Pourhajibagher M, Alaeddini M, Etemad-Moghadam S. et al. Quorum quenching of Streptococcus mutans via the nano-quercetin-based antimicrobial photodynamic therapy as a potential target for cariogenic biofilm. BMC Microbiol 2022; 22 (01) 125
    CrossrefPubMedGoogle Scholar
  • 10 Szafrański SP, Deng ZL, Tomasch J. et al. Quorum sensing of Streptococcus mutans is activated by Aggregatibacter actinomycetemcomitans and by the periodontal microbiome. BMC Genomics 2017; 18 (01) 238
    CrossrefPubMedGoogle Scholar
  • 11 Utamaningyas A, Pramesti HT, Balafif FF. The Streptococcus mutans ability to survive in biofilms and during dental caries formation: scoping review. J Syiah Kuala Dentistry Soc 2022; 18 (01) 238
    Google Scholar
  • 12 Wang X, Li X, Ling J. Streptococcus gordonii LuxS/autoinducer-2 quorum-sensing system modulates the dual-species biofilm formation with Streptococcus mutans. J Basic Microbiol 2017; 57 (07) 605-616
    CrossrefPubMedGoogle Scholar
  • 13 Muras A, Mayer C, Romero M. et al. Inhibition of Streptococcus mutans biofilm formation by extracts of Tenacibaculum sp. 20J, a bacterium with wide-spectrum quorum quenching activity. J Oral Microbiol 2018; 10 (01) 1429788
    CrossrefPubMedGoogle Scholar
  • 14 Asih DW, Widodo ADW, Setiabudi RJ, Sulistiawati M, Tyasningsih W, Wahyu MR. Biofilm formation by the interaction of fungi (Candida tropicalis) with various bacteria. JABET 2022; 6 (01) 84-93
    Google Scholar
  • 15 Wang BY, Kuramitsu HK. Interactions between oral bacteria: inhibition of Streptococcus mutans bacteriocin production by Streptococcus gordonii . Appl Environ Microbiol 2005; 71 (01) 354-362
    CrossrefPubMedGoogle Scholar
  • 16 Wang BY, Deutch A, Hong J, Kuramitsu HK. Proteases of an early colonizer can hinder Streptococcus mutans colonization in vitro. J Dent Res 2011; 90 (04) 501-505
    CrossrefPubMedGoogle Scholar
  • 17 Grela E, Kozłowska J, Grabowiecka A. Current methodology of MTT assay in bacteria - a review. Acta Histochem 2018; 120 (04) 303-311
    CrossrefPubMedGoogle Scholar
  • 18 Parga A, Muras A, Otero-Casal P. et al. The quorum quenching enzyme Aii20J modifies in vitro periodontal biofilm formation. Front Cell Infect Microbiol 2023; 13: 1118630
    CrossrefPubMedGoogle Scholar
  • 19 Chopra A, Bhat SG, Sivaraman K. Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update. J Oral Microbiol 2020; 12 (01) 1801090
    CrossrefPubMedGoogle Scholar
  • 20 Ghods S, Moradali MF, Duryea D, Walker AR, Davey ME. Growth of Porphyromonas gingivalis on human serum albumin triggers programmed cell death. J Oral Microbiol 2022; 15 (01) 2161182
    CrossrefPubMedGoogle Scholar