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CC BY 4.0 · Eur J Dent 2024; 18(04): 1076-1084
DOI: 10.1055/s-0044-1786875
Original Article

Analysis of Tissue Repair of a New Cement Based on Calcium and Strontium Aluminates: A Triple-Blinded, Randomized, Controlled Trial in an Animal Model

Elizandra Silva da Penha
1   Department of Dentistry, Dental School, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Nonato Amorim de Farias Filho
1   Department of Dentistry, Dental School, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Luanna Abílio Diniz Melquíades de Medeiros
1   Department of Dentistry, Dental School, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Rosana Araújo Rosendo
1   Department of Dentistry, Dental School, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Marco Antônio Dias da Silva 
1   Department of Dentistry, Dental School, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Willams Teles Barbosa 
2   SENAI Institute of Innovation (ISI) in Forming and Joining (CIMATEC ISI F&J), Salvador, Bahia, Brazil
,
Raúl García-Carrodeguas
3   Noricum S.L., San Sebastian De Los Reyes, Madrid, Spain
,
Miguel Angel Rodríguez
4   Institute of Ceramics and Glass (ICV-CSIC), Cantoblanco, Madrid, Spain
,
Eliseu Aldrighi Münchow
5   Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
,
Rogério Lacerda-Santos 
6   Department of Orthodontics, Federal University of Juiz de Fora, Governador Valadares, Minas Gerais, Brazil
,
Marcus Vinícius Lia Fook
7   Department of Materials Engineering, Federal University of Campina Grande, Campina Grande, Paraíba, Brazil
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Abstract

Objective The focus of this triple-blind randomized study was to evaluate the biocompatibility of a new root canal filling sealer (RCFS) based on tristrontium aluminate and dodecacalcium hepta-aluminate in living tissue.

Material and Methods Forty-five Wistar rats (Rattus norvegicus) were divided into three groups: control (polyethylene), sealer (Bio-C Sealer, Londrina, PR, Brazil), and experimental (tristrontium aluminate and dodecacalcium hepta-aluminate). The tissues were analyzed under an optical microscope to assess different cellular events at different time intervals (7, 15, and 30 days).

Statistical Analysis Data were analyzed using the Kruskal–Wallis and Dunn (p < 0.05) tests.

Results In the initial period, a moderate inflammatory infiltrate was observed, similar between the endodontic cements groups (p = 0.725). The intensity of the infiltrate decreased with time, with no significant difference among the groups (p > 0.05). The number of young fibroblasts was elevated in all groups evaluated at 7 days. The experimental group showed the highest number of cells at all time intervals, but the difference with the sealer group at 7 (p = 0.001) and 15 days (p = 0.002) and the control group at 30 days was not significant (p = 0.001). Regarding tissue repair events, the amount of collagen fibers increased over the experimental intervals, with no significant difference between the sealer and control groups (p > 0.05).

Conclusion The experimental RCFS based on calcium and strontium aluminates proved to be biocompatible for use in close contact with periapical tissue, inducing a low inflammatory reaction and favoring rapid tissue repair.

Keywords

microscope - biocompatibility - histology - root canal filling sealer

Authors' Contribution

All authors were involved in the work leading to the publication of this paper and have read the paper before this submission. E.S.P., L.A.D.M.M., and R.A.R. conceptualized the study. M.A.D.S., E.A.M., and N.A.F.F. helped in methodology and visualization. R.L.S., M.A.D.S., W.T.B., and M.A.R. were involved in validation and data acquisition. E.S.P., L.A.D.M.M., R.A.R., and M.A.R. helped in original writing. M.V.L.F, R.L.S., E.A.M., W.T.B., and R.G.C. reviewed and edited the manuscript. M.V.L.F, N.A.F.F., and R.G.C. helped in project administration and funding acquisition.




Publication History

Article published online:
22 May 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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