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CC BY 4.0 · Journal of Coloproctology 2024; 44(03): e156-e162
DOI: 10.1055/s-0044-1787141
Original Article

Evaluation of Salvia officinalis in the Treatment of Acetic Acid-induced Ulcerative Colitis in a Rat Model

Authors

  • Reza Shahriarirad 

    1   Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
    2   Thoracic and Vascular Surgery Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

  • Sarvin Seifbehzad

    1   Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

  • Amirhossein Erfani 

    1   Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
    2   Thoracic and Vascular Surgery Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

  • Fatemeh Nekouei

    1   Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

  • Soheil Ashkani-Esfahani 

    3   Department of Orthopedic Surgery, Foot & Ankle Research and Innovation Laboratory (FARIL), Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States

  • Masood Hosseinzadeh

    4   Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran

  • Nader Tanideh

    5   Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

  • Omid Koohi-Hosseinabadi

    6   Center of Comparative and Experimental Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

  • Bahador Sarkari 

    7   Department of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran

Funding The authors declare that they have not received funding from agencies in the public, private, or non-profit sectors for the conduction of the present study.
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Abstract

Introduction Ulcerative colitis (UC) is an inflammatory bowel disease that causes long-lasting inflammation and ulcers within the digestive tract. This study aims to determine the histochemical alteration of Salvia officinalis (sage), an anti-inflammatory and antioxidant herbal agent on UC.

Materials and Methods The disease was induced in 37 Sprague-Dawley rats with 2 mL of 3% acetic-acid (AA) enema. The rats were divided into five groups: a control group (AA), two 5-aminosalicylic (5-ASA) groups treated either orally (AO) or rectally (AR) with a dose of 100 mg/kg, and two salvia groups treated with 300mg/kg salvia orally (SO) or rectally (SR). Histopathological analyses of the colon were done on day 7, and markers such as C-reactive protein (CRP), superoxide dismutase (SOD), and complete blood count were measured.

Result In macroscopic evaluation, the AO group demonstrated the lowest involvement, followed by the SO, SR, AR, and AA groups, respectively (p = 0.01). There was no significant difference between the SO and AO groups (p = 0.10), and the SR and AR groups (p = 0.58). Regarding microscopic histopathological findings, the AO and SO group demonstrated the most satisfactory results, with no significant difference between the AO versus SO, and AR versus SR groups. Inflammation was resolved in all of the AO and SO subjects.

Conclusion Salvia can be beneficial in the treatment course of UC by inhibiting inflammatory responses, increasing the growth and viability of intestinal mucosa, and its antioxidant effects. Therefore, we propose the prescription of salvia as an adds-on or alternative therapy in the management of UC.

Keywords

treatment protocol - salvia officinalis - ulcerative colitis

Ethics Approval and Consent to Participate

The present study was approved by the Medical Ethics Committee of the Shiraz University of Medical Science. The experimental protocol was approved by the Ethics Committee of Shiraz University of Medical Sciences, Shiraz, Iran, and all the criteria for taking care of laboratory animals outlined in the “Guide for the Care and Use of Laboratory Animals” were applied. Ethical code: IR.SUMS.REC.1394.S1075.


Consent for Publication

Not applicable.


Availability of Data and Materials

All data regarding this study has been reported in the manuscript. Please contact the corresponding author if you are interested in any further information.


Authors' Contributions

In this study, the author contributions were as follows: Reza Shahriarirad was in charge of formal analysis, carried out investigative research, and wrote the initial manuscript draft. Sarvin Seifbehzad, Amirhossein Erfani, Fatemeh Nekouei, and Masood Hosseinzadeh contributed to the investigative research as well. Soheil Ashkani-Esfahani played a role in the study's conceptualization and oversaw project administration. Nader Tanideh was instrumental in the research concept and provided overall supervision. Omid Koohi-Hosseinabadi was responsible for developing the methodology and also engaged in investigative research. Lastly, Bahador Sarkari acted as a supervisor and was in charge of project administration.




Publication History

Received: 09 March 2024

Accepted: 09 April 2024

Article published online:
22 July 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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