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CC BY 4.0 · Rev Bras Ortop (Sao Paulo) 2024; 59(04): e519-e525
DOI: 10.1055/s-0044-1787768
Artigo Original
Básica

Histomorphometric Study of Non-critical Bone Defect Repair after Implantation of Magnesium-substituted Hydroxyapatite Microspheres

Artikel in mehreren Sprachen: português | English
Jacqueline de Azerêdo Silva 
1   Centro de Medicina Hiperbárica do Nordeste (CMHN), Salvador, BA, Brasil
,
George Gonçalves dos Santos 
2   Centro de Ciências da Saúde (CCS), Universidade Federal do Recôncavo da Bahia (UFRB), Santo Antônio de Jesus, BA, Brasil
,
Iorrana Índira dos Anjos Ribeiro 
3   Programa de Pós-Graduação em Processos Interativos dos Órgãos e Sistemas (PPGPIOS), Faculdade Adventista da Bahia (FADBA), Cachoeira, BA, Brasil
,
Ana Maria Guerreiro Braga da Silva 
4   Centro de Ciências Agrárias, Ambientais e Biológicas (CCAAB), Universidade Federal do Recôncavo da Bahia (UFRB), Cruz das Almas, BA, Brasil
,
Isabela Cerqueira Barreto 
5   Instituto de Ciências da Saúde (ICS), Universidade Federal da Bahia (UFBA), Salvador, BA, Brasil
,
Marcos Almeida Matos 
6   Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, BA, Brasil
,
Maurício Andrade Barreto 
6   Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, BA, Brasil
,
Fúlvio Borges Miguel 
5   Instituto de Ciências da Saúde (ICS), Universidade Federal da Bahia (UFBA), Salvador, BA, Brasil
› Institutsangaben Financial Support The authors declare that they have not received financial support from agencies in the public, private, or non-profit sectors to conduct the present study.
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Abstract

Objective The present study aims to analyze histomorphometrically the repair of a non-critical bone defect after implantation of hydroxyapatite (HA) microspheres substituted by magnesium (Mg).

Methods Thirty rats were distributed into 3 experimental groups, evaluated at 15 and 45 days postoperatively: HAG (bone defect filled with HA microspheres); HAMgG (bone defect filled with HA microspheres replaced with 1 mol% Mg), and CG (bone defect without implantation of biomaterials).

Results After 15 days, the biomaterials filled the entire defect extent, forming a new osteoid matrix between the microspheres. In the CG, this neoformation was restricted to the edges with the deposition of loose connective tissue with reduced thickness. At 45 days, new bone formation filled almost the entire extension of the bone defect in the 3 groups, with statistically significant osteoid deposition in the CG despite the reduced thickness compared with the HAG and HAMgG. The groups with biomaterial implantation displayed a more abundant osteoid matrix than at 15 days.

Conclusion The biomaterials studied showed biocompatibility, osteoconductivity, and bioactivity. The Mg concentration in the substituted HA did not stimulate more significant bone formation than HA without this ion.

Keywords

biomaterials - bone and bones - bone regeneration - hydroxyapatite - magnesium

Authors' Contributions

The authors contributed individually and significantly to the development of this article: JAS: Study conception and design; manuscript preparation; and surgical procedures. GGS: Manuscript preparation; and surgical and technical procedures. IIAR: Manuscript preparation; and surgical and technical procedures. AMGBS: Manuscript preparation and english text review. ICB: Surgical and technical procedures and histopathological analysis. MAM: Study conception and design; and histopathological analysis. MAB: Study conception and design; and histopathological analysis. FBM: Study conception and design; surgical and technical procedures; and critical review.


Work carried out at the Department of Orthopedics and Traumatology, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil.




Publikationsverlauf

Eingereicht: 04. Oktober 2023

Angenommen: 05. April 2024

Artikel online veröffentlicht:
04. September 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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