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CC BY-NC-ND 4.0 · South Asian J Cancer
DOI: 10.1055/s-0044-1788050
Original Article

Evaluation of Applicability of Tumor Budding and Poorly Differentiated Clusters as Additional Prognostic Markers in Colorectal Cancers

Sagarika Sarkar
1   Department of Pathology, Employees State Insurance Post Graduate Institute of Medical Sciences and Research (ESI PGIMSR), Manicktala, Kolkata, West Bengal, India
,
Rena Guha
2   Department of Pathology, Prafulla Chandra Sen Government Medical College and Hospital, Arambagh, West Bengal, India
,
Sudipta Chakrabarti 
3   Department of Pathology, Employees State Insurance Corporation (ESIC) Medical College, Bihta, Patna, Bihar, India
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Abstract

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Sagarika Sarkar

Purpose Very few studies have assessed tumor budding (TB) and poorly differentiated cell clusters (PDCs) simultaneously in colorectal cancers (CRCs). The goal of this study was to establish a correlation between these two pertinent histological features and to reinforce the importance of their incorporation in routine histopathological reporting of CRC cases as a means to predict clinical outcome.

Methods Resection specimens of colorectal carcinoma were included in the study. Patients who received presurgical therapy, or refused consent were excluded. PDC and TB were evaluated in routine hematoxylin and eosin-stained histopathological sections taken from the advancing edge of the tumor. TB and PDC were reported by selecting a “hotspot” chosen after review of all available slides with invasive tumor. It was then followed by their correlation with other known prognostic factors.

Results Spearman's rho calculator for strength of association between TB and PDC as well as association of TB and PDC individually with known prognostic factors revealed statistical significance. Correlation of TB and PDC with histologic grade, primary tumor (pT), and regional lymph node (pN) stage was done based on one-way analysis of variance calculator, which yielded statistically significant results.

Conclusion Evaluation of these two histological parameters in the same hotspot field at the tumor invasive front plays a fundamental role in the definition of cancer aggressiveness and prediction of tumor behavior.

Keywords

colorectal cancers (CRC) - morphology - tumor budding (TB) - poorly differentiated cell cluster (PDC) - prognostic markers

Authors' Contributions

S.S. helped in study conception and design, material preparation, data collection, and analysis, written first draft of the manuscript; whereas R.G. and S.C. was involved in study conception and design, material preparation, data collection, and analysis.


Informed Consent

Informed consent was obtained from all participants included in the study.


Ethics Approval

The Institutional Ethics Committee has approved the study.




Publication History

Received: 15 July 2023

Accepted: 11 June 2024

Article published online:
03 July 2024

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