Abstract
Coronary artery disease (CAD) is one of the most common cardiovascular diseases (CVDs),
being the foremost reason for mortality and disability globally. It is a cascade of
polygenic architecture of various disorders as a resultant of complexities between
various genetic factors and environmental factors. The aim of the present study was
to investigate whether the ET1 and APE1 gene polymorphism is associated with the susceptibility to CAD. This study was approved
by the Ethical Review Committee of Era Medical College and Hospital. Three milliliters
of venous blood sample was collected in ethylenediaminetetraacetic acid-coated vials,
and genomic DNA was isolated by using the standard phenol-chloroform extraction method
for restriction fragment length polymorphism-polymerase chain reaction study. The
APE1 gene AA, AG, GG, and AG + GG genotypes frequencies were 13.91, 40.87, 45.22, and
86.99% in CAD cases and 13, 50, 37, and 87% in controls, respectively. The ET1 gene GG, GT, TT, GT + TT genotypes frequencies were 6.67, 37.5, 55.8, and 93.34%
in CAD cases and 6.67, 25.33, 68, and 93.34% in controls. The ET1 and APE1 gene polymorphisms were not significantly associated with the risk of CAD.
Keywords
coronary artery disease - endothelin 1 - apurinic/apyrimidinic endonuclease 1 - gene
polymorphism
