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CC BY 4.0 · Journal of Coloproctology 2024; 44(03): e163-e170
DOI: 10.1055/s-0044-1788602
Original Article

The Predictive Value of hsa_circ_0001313 (circCCDC66) in Egyptian Rectal Cancer Patients: A New Era in Precision Medicine

Hend M. Batea 
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Egypt
,
Safaa H. Mohy El-dine
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Egypt
,
Eman M. Kamha
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Egypt
,
Gehan M. Khedr
2   Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Alexandria University, Egypt
,
Ahmed Moaz
3   Department of Colorectal Surgery, General Surgery Unit, Faculty of Medicine, Alexandria University, Egypt
,
Doaa A. Abdelmonsif
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Egypt
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Funding The authors declare that they have not received funding from agencies in the public, private or nonprofit sectors to conduct the present study.
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Abstract

Introduction The involvement of circular RNAs (circRNAs) in cancer research has been emphasized in recent years due to evidence of their involvement in malignancy pathogenesis. Yet, the involvement of circRNAs in the resistance to cancer treatment remains to be clarified. Circular RNA 0001313 (circ_0001313) has a distinct expression in different cancers, and it is overexpressed in rectal cancer; hence, it could be a promising non-invasive stable biomarker and a therapeutic target for rectal cancer. Yet, its predictive role has not been studied in Egyptian rectal cancer patients.

Objective To study the predictive value of circulating circ_0001313 (circ_CCDCC6) in assessing the response to neoadjuvant chemoradiotherapy (nCRT) in patients with rectal cancer and its relation to radiological and pathological response.

Materials and Methods The present study included 50 patients with locally advanced rectal cancer and 20 healthy subjects as controls. The analysis of the relative circ_CCDCC6 expression was performed using the real-time quantitative polymerase chain reaction (qPCR) method.

Results The circ_CCDCC6 was found to be significantly more expressed in rectal cancer patients compared with controls (p < 0.001). Moreover, its expression level was significantly higher in nonresponders to nCRT compared with responders (p < 0.001). Furthermore, a receiver operating characteristic (ROC) curve analysis was performed to evaluate the predictive performance of circulating circ_0001313; its sensitivity in predicting the response to treatment was of 93.33%, and its specificity was of 91.43%.

Conclusion Significant up-regulation of circ_0001313 in rectal cancer suggests a potential oncogenic role, and higher expression of circ_0001313 among nonresponders suggests that it could be a predictor of the response to nCRT.

Keywords

circ_0001313 - circular RNA - rectal cancer - gene expression - response to nCRT

Authors' Contributions

All authors contributed equally to; data collection, implementation of the experiment, analysis and interpretation of results. The first draft of the manuscript was written and edited by Hend M Batea, Doaa A Abdelmonsif, Gehan M Khedr, and Ahmed Moaz. Safaa H Moy El-dine and Eman M Kamha reviewed the manuscript and approved the final version.




Publikationsverlauf

Eingereicht: 21. März 2024

Angenommen: 06. Mai 2024

Artikel online veröffentlicht:
13. August 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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