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CC BY 4.0 · Avicenna J Med
DOI: 10.1055/s-0045-1802666
Original Article

CD11b Expression Level Measurement to Distinguish Asthma Phenotype in Pediatric Patients

Tien Budi Febriani
1   Department of Pediatric, Faculty of Medicine, Universitas Islam Indonesia, Yogyakarta, Indonesia
,
Soeroyo Mahfudz
1   Department of Pediatric, Faculty of Medicine, Universitas Islam Indonesia, Yogyakarta, Indonesia
,
Emi Azmi Choironi
1   Department of Pediatric, Faculty of Medicine, Universitas Islam Indonesia, Yogyakarta, Indonesia
,
Ninda Devita
2   Department of Microbiology, Faculty of Medicine, Universitas Islam Indonesia, Yogyakarta, Indonesia
,
Adika Zhulhi Arjana 
3   Department of Clinical Pathology, Faculty of Medicine, Universitas Negeri Yogyakarta, Yogyakarta, Indonesia
,
Muhammad Nur Imansyah
4   Faculty of Medicine, Universitas Negeri Yogyakarta, Yogyakarta, Indonesia
› Author Affiliations Funding The study was funded by a research grant from the Faculty of Medicine, Universitas Islam Indonesia, in 2018. The funding body had no role in the design of the study, collection, analysis, or interpretation of data, or in the writing of the manuscript. The authors affirm that the research was conducted independently and without any influence from the funding source.
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Abstract

Background Inflammation is involved in asthma pathogenesis. Based on the majority of inflammatory cells found in blood samples, asthma is divided into neutrophilic and eosinophilic phenotype. CD11b is a leukocyte surface antigen that is involved in the inflammation process. However, the immunologic response mechanism in asthma phenotypes, especially in pediatric population, is still unclear. The aim of this study was to find the difference of CD11b expression in neutrophilic and eosinophilic phenotype of asthma in pediatric patients.

Methods This is an analytic nonexperimental study. Data are gathered with cross-sectional method. Study subject is pediatric patients (5–17 years old) with asthma in Persaudaraaan Djamaah Hadji Indonesia and Wonosari hospitals. Blood samples for leukocyte differential count were obtained from patients who met the inclusion and exclusion criteria. Samples were analyzed with flow cytometry to measure the CD11b level. The obtained data was analyzed with independent t-test with Medcalc software.

Result A total of 40 patients were enrolled in this study. Based on blood sample analysis, 70% patients had neutrophilic asthma and 30% patients had eosinophilic asthma. There was no significant difference between patient ages mean in both groups (7.071 vs. 7.471; p = 0.7186). The mean of leukocyte count was significantly higher in patients with neutrophilic asthma, compared with eosinophilic asthma (8.468 vs. 4.817 × 109/L; p = 0.0008). CD11b expression in neutrophilic asthma was significantly higher than eosinophilic asthma (p = 0.046). No association was found between CD11b expression levels with age, sex, and body mass index.

Conclusion CD11b expression level is higher in neutrophilic phenotype compared with the eosinophilic phenotype in pediatric asthma patients. Its increased expression could provide insights into the mechanisms driving airway inflammation and remodeling. This marker might be explored as a target for novel therapies aimed at modulating immune activation to reduce asthma exacerbations and improve long-term outcomes.

Keywords

asthma phenotype - pediatric asthma - CD11b

Authors' Contributions

T.B.F.: Designed the study and wrote the paper.

S.M.: Designed the study and wrote the paper.

E.A.C.: Designed the study and wrote the paper.

N.D.: Analyzed the data and wrote the paper.

A.Z.A.: Collected the data and wrote the paper.

M.N.I.: Collected the data.




Publication History

Article published online:
02 April 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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