Nitric Oxide/cGMP Mediates the Spasmolytic Action of 3,4′-Dihydroxy-5,5 ′-dimethoxybibenzyl from Scaphyglottis livida

Samuel Estrada; Alejandra Rojas; Yaira Mathison; Anita Israel; Rachel Mata

doi:10.1055/s-1999-14056

Planta Medica, Table of Contents

Planta Med 1999; 65(2): 109-114
DOI: 10.1055/s-1999-14056

Original Paper

Georg Thieme Verlag Stuttgart · New York

Nitric Oxide/cGMP Mediates the Spasmolytic Action of 3,4^′-Dihydroxy-5,5^′-dimethoxybibenzyl from Scaphyglottis livida [*]

Samuel Estrada¹ , Alejandra Rojas² , Yaira Mathison^3,4 , Anita Israel⁴ , Rachel Mata¹

¹Departamento de Farmacia, Facultad de Química and Unidad de Investigación en Plantas Medicinales, Instituto de Química, Universidad Nacional Autónoma de México, México
²Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, México
³Facultad de Medicina José María Vargas and
⁴Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela

Abstract

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Abstract:

Five aromatic compounds, 3,4^′-dihydroxy-5,5^′-dimethoxybibenzyl (1), batatasin III (2), coelonin (3), 3,7-dihydroxy-2,4-dimethoxyphenanthrene (4), and 3,7-dihydroxy-2,4,8-trimethoxyphenanthrene (5) were isolated from the orchid Scaphyglottis livida (Lindley) Schltr. Compounds 1 -5 induced a concentration-dependent inhibition of the spontaneous contractions of the rat ileum with potencies comparable or higher to that of papaverine. The relaxation evoked by compounds 1-4 was blocked by _L-NAME, an inhibitor of nitric oxide synthase. It was also demonstrated that 1 increased cyclic GMP content in rat ileum rings. Compound 1-induced elevation of cGMP was inhibited by _L-NAME and ODQ, inhibitors of nitric oxide synthase and soluble guanylyl cyclase, respectively. These results indicate that nitric oxide/cGMP formation constitute the signaling pathway in the spasmolytic action of compound 1.

Key words:

Scaphyglottis livida - Orchidaceae - phenanthrenes - dihydrostilbenes - smooth muscle relaxation - nitric oxide - _L-NAME - ODQ - soluble guanylyl cyclase

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References

1 Chemical Studies on Mexican Plants Used in Traditional Medicine XXXIX. Taken in part from the PhD thesis of S. Estrada.

Nitric Oxide/cGMP Mediates the Spasmolytic Action of 3,4′-Dihydroxy-5,5 ′-dimethoxybibenzyl from Scaphyglottis livida [*]

Nitric Oxide/cGMP Mediates the Spasmolytic Action of 3,4^′-Dihydroxy-5,5^′-dimethoxybibenzyl from Scaphyglottis livida [*]