Subscribe to RSS
DOI: 10.1055/s-1999-14083
Georg Thieme Verlag Stuttgart · New York
In Vitro Antisickling Activity of a Rearranged Limonoid Isolated from Khaya senegalensis
Publication History
June 12, 1998
November 8, 1998
Publication Date:
31 December 1999 (online)

Abstract:
We previously observed that aqueous extracts of the stem bark and leaves of Khaya senegalensis exhibited a strong antisickling activity. These results prompted us to find out the constituent(s) responsible for these properties using an in vitro bio-guided fractionation. The bioassay was based on sickle cells countings, before and after deoxygenation, in blood samples taken from patients with severe sickle cell anemia and pre-incubated with the drugs to be tested. The main active constituent was identified as a rearranged limonoid whose structure was recently elucidated. In comparison with pentoxifylline used as standard, the in vitro antisickling activity of this limonoid was much higher at any concentrations and incubation conditions. In addition, it did not alter significantly the corpuscular indices.
Key words:
Khaya senegalensis - Meliaceae - rearranged limonoid - antisickling activity - corpuscular indices