Abstract:
The effect of an Egyptian medicinal plant, Cleome droserifolia (Forssk.)
Del. on nitric oxide (NO) produrtion in bacillus Calmette-Cuerin-induced moiise
peritoneal macrophages arti-vated by lipopolysaccharide was investigated in
vitro. The methanoi extract of C. droserifolia reduced
the NO produrtion, and two flavonoids were isolated as the active components.
The new one was determined to be 5,4′-dihydroxy-6,7,8,3
′,5′-pentamethoxyflavone (
1) and the other was identified as 5,4′-dihydroxy-6,7,8,3
′-tetramethoxyflavone (8-methoxycirsilineol;
2). Compound 1 concentration-dependently suppressed
the NO production and was effective at a non-toxic concentration (12.5 μ/ml).
The suppressive activity of 2 was weaker than that
of 1.
Key words:
Cleome droserifolia
- Capparidaceae - flavonoid - nitric oxide - inflammation - macrophage
