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Synlett 1999; 1999(10): 1667-1678
DOI: 10.1055/s-1999-2914
new tools in synthesis
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Fluorescent Oligonucleotides - Versatile Tools as Probes and Primers for DNA and RNA Analysis

Christian Wojczewski* , Karen Stolze, Joachim W. Engels
  • *Institut für Organische Chemie, Johann Wolfgang Goethe-Universität, Marie-Curie-Str. 11, 60439 Frankfurt am Main, Germany; Fax (+ 49)69 79 82 91 48; E-mail: engels@ews1.org.chemie.uni-frankfurt.de
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Publication Date:
31 December 1999 (online)

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Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre- and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes.

fluorescent oligonucleotides - automated solid-phase synthesis - modified nucleosides - fluorescence spectroscopy - DNA