Molecular Analysis of Implantation

ABSTRACT

The great frontier in reproductive medicine is implantation biology. The scientific understanding of normal implantation lags behind what is known in areas such as fertilization. For obvious reasons this process is difficult to analyze. Recent investigations utilizing molecular biology approaches have scratched the surface of the interaction between the developing trophoblast and the receptive endometrium. This article describes these approaches with techniques such as serial analysis of gene expression (SAGE) and complementary DNA array hybridization, which will soon reveal the key mediators of normal implantation and result in an understanding of failed implantation.

KEYWORD

molecular biology - endometrium - trophoblast - genetics

REFERENCES

• 1 Yi X J, Jiang H Y, Lee K KH. Expression of vascular endothelial growth factor (VEGF) and its receptors during embryonic implantation in the golden hamster (Mesocricetus auratus).  Cell Tissue Res . 1999;  296 339-349
• 2 Cowan B D, Hines R S, Case S T, Brackin M N. Temporal and cell-specific gene expression by human endometrium after co-culture with trophoblast.  Am J Obstet Gynecol . 1999;  180 806-814
• 3 Hines R S, Brackin M N, Cowan B D. Differential display demonstrates in vitro regulation of specific endometrial messenger ribonucleic acid (mRNA) by trophoblast secretory products. American Society for Reproductive Medicine, 53rd Annual Meeting, Cincinnati, October 1997
• 4 Nie G Y, Li Y, Hampton A L, Salamonsen L A, Clements J A. Identification of monoclonal nonspecific suppressor factor beta (mNSF-β) as one of the genes differentially expressed at implantation sites compared to interimplantation sites in the mouse uterus.  Mol Reprod Dev . 2000;  55 351-363
• 5 Velculescu V E, Zhang L, Vogelstein B, Kinzler K W. Serial analysis of gene expression.  Science . 1995;  270 484-487
• 6 Madden S l, Galella E A, Zhu J, Bertelsen A H, Beaudry G A. SAGE transcript profiles for p53-dependent growth regulation.  Oncogene . 1997;  15 1079-1085
• 7 Nacht M, Ferguson A T, Zhang W. Combining serial analysis of gene expression and array technologies to identify genes differentially expressed in breast cancer.  Cancer Res . 1999;  59 5464-5470
• 8 Hines R S, Brackin M N, Case S T, O'Dell A, Cox C K, Cowan B D. Alteration of steady state endometrial mRNA by co-culture with trophoblast. Society for Gynecologic Investigation, Atlanta, No. 147, p. 83A, 1999
• 9 Okulicz W C, Ace C I. Progesterone-regulated gene expression in the primate endometrium.  Semin Reprod Endocrinol . 1999;  17 241-255
• 10 Mitsunari M, Harada T, Tanikawa M, Iwabe T, Taniguchi F, Terakawa N. The potential role of stem cell factor and its receptor c-kit in the mouse blastocyst implantation.  Mol Hum Reprod . 1999;  5 874-879
• 11 Taga M, Ikeda M, Shigeta H, Hiraga M. Gene expression of double-standard RNA-dependent protein kinase in the human endometrium and decidua.  Mol Cell Endocrinol . 1999;  158 65-68
• 12 Auernhammer C J, Memed S. Leukemia-inhibitory factory: neuroimmune modulator of endocrine function.  Endocr Rev . 2000;  21 313-345
• 13 Stewart C L, Kaspar P, Brunet L J. Blastocyst implantation depends on maternal expression of leukemia inhibitory factor.  Nature . 1992;  359 76-79
• 14 Charnock-Jones D S, Sharkey A M, Fenwoick P, Smith S K. Leukemia inhibitory factor mRNA concentration peaks in human endometrium at the time of implantation and the blastocyst contains mRNA for the receptor at this time.  J Reprod Fertil . 1994;  101 421-426
• 15 Giess R, Tanasescu I, Steck T, Sendtner M. Leukaemia inhibitory factory gene mutations in infertile women.  Mol Hum Reprod . 1999;  5 581-586
• 16 Taniguchi F, Harada T, Yoshida S. Paracrine effects of bFGF and KGF on the process of mouse blastocyst inplantation.  Mol Reprod Dev . 1998;  50 54-62
• 17 Koji T, Chedid M, Rubin J S. Progesterone-dependent expression of keratinocyte growth factor mRNA in stromal cells of the primate endometrium: keratinoctye growth factor as a progestomedin.  J Cell Biol . 1994;  125 393-401
• 18 Siegfried S, Pekonen F, Nyman T, Ammala M. Expression of mRNA for keratinocyte growth factor and its receptor in human endometrium.  Acta Obstet Gynecol Scand . 1995;  74 410-414
• 19 Slayden O D, Rubin J S, Lacey D L, Brenner R M. Effects of keratinocyte growth factor in the endometrium of rhesus macaques during the luteal-follicular transition.  J Clin Endocrin Metab. 85 275-285