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DOI: 10.1055/s-2000-8553
Antiplatelet Aggregation Principles from Ephemerantha lonchophylla
Publication History
Publication Date:
31 December 2000 (online)
Abstract
Bioactivity-directed separation led to the identification of four compounds, viz. denbinobin (1), 3,7-dihydroxy-2,4-dimethoxyphenanthrene (2), 3-methylgigantol (3), and erianthridin (4) from the ethanolic extract of Ephemerantha lonchophylla. Antiplatelet tests were carried out using 4 different aggregation inducers, viz. arachidonic acid (AA), thrombin, collagen and platelet activating factor (PAF). The results indicated that only compounds 2, 3, and 4 exhibited generally significant anti-aggregation activities with that against AA-induced aggregation being most effective. Estimated IC50, values in this regard for 2, 3, and 4 were 24 μM, 30 μM and 9 μM, respectively.
References
-
1 Hsu H Y,, Chen Y P,, Sheu S J,, Hsu C S,, Chen C C,, Chang H C.. Oriental Materia Medica - a Concise Guide, 1986, p. 582, Oriental Healing Arts Institute, Los Angeles.
- 2 Ma G X,, Xu G J,, Xu L S,, Wang Z T.. Microscopic identification of the Chinese drug Shihu IV. J. of China Pharmaceutical University. 1995;; 26 134-8
- 3 Chen C C,, Wu L G,, Ko F N,, Teng C N.. Antiplatelet aggregation principles of Dendrobium loddigesii. . J. Nat. Prod.. 1994;; 57 1271-4
- 4 Chen H Y,, Shiao M S,, Huang Y L,, Shen C C,, Lin Y L,, Kuo Y H et al.. Antioxidant principles from Ephemerantha lonchophylla. . J. Nat. Prod.. 1999;; 62 1225-7
- 5 Tezuka Y,, Hirano H,, Kikuchi T,, Xu G J.. Constituents of Ephemerantha lonchophylla, isolation and structure elucidation of new phenolic compounds, ephemeranthol-A, ephemeranthol-B, and ephemeranthoquinone, and of a new diterpene glucoside, ephemeranthoside. Chem. Pharm. Bull.. 1991;; 39 593-8
- 6 Leong Y W,, Kang C C,, Harrison L J,, Powell A D.. Phenanthrenes, dihydrophenanthrenes and bibenzyls from the orchid Bulbophyllum vaginatum. . Phytochemistry. 1997;; 44 157-65
- 7 Vargaftig B B,, Chignard M,, Benveniste J.. Present concepts on the mechanisms of platelet aggregation. Biochem. Pharmacol.. 1981;; 30 263-71
- 8 Vane J R.. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nature-New Biology. 1971;; 231 232-5
- 9 Siess W.. Molecular mechanisms of platelet activation. Physiological Review. 1989;; 69 58-178
- 10 Chen C C,, Huang Y L,, Ou J C,, Su M J,, Yu S M,, Teng C M.. Bioactive principles from the roots of Lindera megaphylla. . Planta Medica. 1991;; 57 406-8
- 11 Born G VR,, Cross M J.. The aggregation of blood platelet. J. Physiol.. 1963;; 168 178-95
Prof. Chien-Chih Chen
National Research Institute of Chinese Medicine
No. 155 - 1, Sec. 2, Li Nung St., Peitou
Taipei 11221
Taiwan
Republic of China
Email: ccchen@cma23,nricm.edu.tw
Phone: +886 - 2 - 28264276