Abstract
Nitric oxide (NO) and tumor necrosis factor-α (TNF-α) are the major mediators produced
in activated macrophages which contribute to the circulatory failure associated with
septic shock. An activity-guided fractionation of an MeOH extract of stem bark of
Magnolia obovata afforded two inhibitors of NO production in lipopolysaccharides (LPS)-activated macrophages
by the suppression of i-NOS expression. Their structures were elucidated by spectroscopic
methods to be magnolol and honokiol with IC50 values of 16.8 and 6.4 μM, respectively. They also inhibited the production of TNF-α
in LPS-activated macrophages. Thus, these compounds may be possible candidates for
the development of new drugs to treat endotoxemia accompanied by the overproduction
of NO and TNF-α.
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Prof. Jae-Ha Ryu
College of Pharmacy Sookmyung Women's University
Chungpa-Dong 2-Ka
Yongsa-Ku
Seoul 140-742
Korea
eMail: ryuha@sdic.sookmyung.ac.kr
Telefon: +82-2-714-0745
Fax: +82-2-710-9568