Anti-Inflammatory Activity of Dichloromethane Extract of Heterotheca inuloides in Vivo and in Vitro* []

Abstract

The dichloromethane extract from the dried flowers of Heterotheca inuloides Cass. was investigated on several pharmacological models of inflammation in vivo and in vitro. It showed anti-inflammatory activity on the croton oil-induced oedema test in mouse ear, at 1 mg/ear. The compound isolated from this extract, 7-hydroxy-3,4-dihydrocadalin, showed anti-inflammatory effect on the same experimental model (ED₅₀ of 0.9 μmol/ear), as well as on COX-1 and COX-2 catalysed prostaglandin biosynthesis assays, with IC₅₀ values of 22 μM and 526 μM, respectively. No effect was observed on carrageenan-induced oedema and on fMLP/PAF-induced exocytosis of human neutrophils. The COX-1 inhibitory effect showed by 7-hydroxy-3,4-dihydrocadalin might be related to the anti-inflammatory activity on the topical oedema induced by croton oil.

1 * Part of the results were presented at the 5^th Joint Meeting of the PSE, GA, ASP and AFERP: 2000 Years of Natural Product Research - Past, Present and Future. Amsterdam, The Netherlands (1999).

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1 * Part of the results were presented at the 5^th Joint Meeting of the PSE, GA, ASP and AFERP: 2000 Years of Natural Product Research - Past, Present and Future. Amsterdam, The Netherlands (1999).