Planta Med 2000; 66(6): 506-510
DOI: 10.1055/s-2000-8614
Original Paper
Georg Thieme Verlag Stuttgart · New York

Effects of Triterpenoids from Pueraria lobata on Immunohemolysis: β-D-Glucuronic Acid Plays an Active Role in Anticomplementary Activity in Vitro

Sei-Ryang Oh1 , Junei Kinjo2 , Yuichi Shii2 , Tuyoshi Ikeda2 , Toshihiro Nohara2 , Kyung Seop Ahn1 , Jung Hee Kim1 , Hyeong-Kyu Lee1,*
  • 1 Immunomodulator Research Lab., Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea
  • 2 Faculty of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
Further Information

Publication History

Publication Date:
31 December 2000 (online)

Abstract

The anticomplementary properties of kaikasaponin III (4) and soyasaponin I (8) from Pueraria lobata and their hydrolytic analogs were investigated in vitro. Diglycosidic saponins [kaikasaponin I (3), soyasaponin III (7)] showed most potent anticomplementary activities, followed by monoglycosidic saponins [soyasapogenol B monoglucuronide (6), sophoradiol monoglucuronide (2)] and triglycosidic saponins [soyasaponin I (8), kaikasaponin III (4)], whereas sophoradiol (1) and soyasapogenol B (5) showed enhancement of hemolysis under the presence of serum on the classical pathway of complement system. But all of them showed very weak or no anticomplementary activities on the alternative pathway of complement system. The anticomplementary activity of the saponins was influenced by the nature of glucuronic acid, where the free acid forms (-COOH) showed much more potent activity than the sodium salt forms (-COO - Na+) or methyl ester forms (-COOCH3), and the reduced forms (-CH2OH) decreased the activity significantly.

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Dr. Hyeong-Kyu Lee

Immunomodulator Research Lab. Korea Research Institute of Bioscience and Biotechnology

Yusong P.O. Box 115

Taejon 305-600

Korea

Email: hykylee@mail.kribb.re.kr

Phone: +82-42-860-4608

Fax: +82-42-860-4413