New Lanostanoids from Ganoderma lucidum that Induce NAD(P)H:Qui-none Oxidoreductase in Cultured Hepalclc7 Murine Hepatoma Cells

Abstract

Two new lanostanoids were isolated from the basidiocarp of Ganoderma lucidum and were identified as 26,27-dihydroxy-5α-lanosta-7,9(11),24-triene-3,22-dione (1) and 26-hydroxy-5α-lanosta-7,9(11),24-triene-3,22-dione (2) by their respective spectral data. Crude extracts and the isolated compounds were tested for their potential to induce NAD(P)H:quinone oxidoreductase (QR), a phase 2 drug-metabolizing enzyme, as an approach to detect potential cancer chemopreventive activity. Compound 2 doubled the specific activity of QR at a concentration of 3.0 μg/ml, whereas compound 1 was significantly less active (1.7-fold induction at 20 μg/ml). In addition, both compounds weakly inhibited sheep vesicle cyclooxygenase 1 activity at a test concentration of 40 μg/ml.

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