Mitogenic Effect of Parkia speciosa Seed Lectin on Human Lymphocytes

Abstract

Mitogenic activity of a lectin, purified from Parkia speciosa seeds, on the isolated peripheral blood lymphocytes taken from normal blood donors and patients with esophageal carcinoma was examined using [³ H]thymidine incorporation. The lectin increases the incorporation of [³ H]thymidine into DNA of human lymphocytes. The activity of the lectin increased as its concentration was increased and then declined once the concentration passed an optimum point. The stimulant effect was also expressed using a proliferation index (PI): the ratio of [³ H]thymidine incorporated into lymphocytes in the presence and absence of the lectin. The mitogenic activity of the lectin is comparable to those of the known T-cell mitogens, such as concanavalin A, phytohaemagglutinin, and pokeweed mitogen. Only slightly less responsiveness was observed in the case of lymphocytes from esophageal cancer compared to lymphocytes from normal donors.

Parkia speciosa - Leguminosasae - lectin - mitogen - lymphocyte proliferation

References

• 1 Suvachittanont  W,, Pothirakit  P.. Protein in Parkia speciosa seeds.  Songkla Med. J.. 1988;;  6 23-30
• 2 Tsuda  M,, Kurashima  Y.. Nitrite-trapping capacity of thioproline in the human body. In: IARC Scientific Publication No. 105. International for Research on Cancer, Lyon,; 1991: 123-8
• 3 Suvachittanont  W,, Kurashima  Y,, Hiroyasu  E,, Tsuda  M.. Formation of thiazolidine-4-carboxylic acid (thioproline), an effective nitrite-trapping agent in human body, in Parkia speciosa seeds and other edible leguminous seeds in Thailand.  Food Chemistry. 1996;;  55 359-63
• 4 Suvachittanont  W,, Peutpaiboon  A.. Lectin from Parkia speciosa seeds.  Phytochemistry. 1992;;  31 4065-70
• 5 Hsu  S M,, Ree  H J.. Histochemical studies on lectin binding in reactive lymphoid tissues.  J. Histochem. and Cytochem.. 1983;;  31 538-46
• 6 Readler  A,, Readler  E J.. The use of lectins to study normal differentiation and malignant transformation.  J. Cancer Res. and Clinical Oncol.. 1985;;  109 245-51
• 7 Orita  K,, Miwa  H,, Fukada  H,, Yumura  M,, Uchida  Y,, Mamnami  T et al.. Preoperative cell-mediated immune status of gastric cancer patients.  Cancer. 1976;;  38 2343-8
• 8 Humphrey  L J,, Humphrey  M A,, Singla  O,, Volenic  F J.. Immunologic responsiveness of patients with cancer. Relationship to tumor type, stage, and prognosis.  Ann. Surg.. 1981;;  5 574-8
• 9 Kilpatric  D C,, Yeoman  M M.. Purification of lectin from Datura stratonium. .  Biochem. J.. 1978;;  175 1151-3
• 10 Kaver  I,, Pecth  M,, Trainin  N,, Greenstein  A,, Braf  Z.. T Lymphocytes subsets and function in peripheral blood of patients with urological cancer.  Oncology. 1992;;  49 108-13
• 11 Prujansky  A,, Ravid  A,, Sharon  N.. Cooperativity of lectin binding to lymphocytes, and its relevance to mitogenic stimulation.  Biochim. Biophys. Acta. 1978;;  508 147-56
• 12 Douglas  S D,, Kamin  R M,, Fundenberg  H H.. Human lymphocyte response to phytomitogens in vitro: normal, agammaglobulinaemic and paraproteinemic individuals.  J. Immunol.. 1969;;  103 1185-95
• 13 Paus  E,, Steen  H B.. Mitogenic effect of α-mannosidase on lymphocytes.  Nature. 1978;;  272 452-4
• 14 Novogrodsky  A,, Katchalski  E.. Mitogenic effect of glycosidase on lymphocytes.  Proc. Natl. Acad. Sci. U.S.A.. 1973;;  70 1824-7
• 15 Novogrodsky  A,, Lotan  A,, Ravid  A,, Sharon  N.. Mitogenic effect of α-mannosidase on lymphocytes.  J. Immunol.. 1975;;  115 1243-8
• 16 Kilpatrick  D C.. Isolation of lymphocytes and accessory cells for assays of mitogenicity. In: Gabius H-J, Gabius S, editors Lectin and Glycobiology. Springer Verlag Berlin; 1993: 369-75
• 17 Broh'ee  D,, Vanhaeverbeek  M,, Kennes  B,, Ne've  P.. Multilinear regression analysis of the lymphocytes response to lectin mitogens as related to the subjects' age and the subsets of cultured peripheral blood mononuclear cells.  Med. Sci. Res.. 1990;;  18 639-41
• 18 Mueller  D L,, Jenkins  M K,, Chiodetti  L,, Schwartz  R.. An intracellular calcium increase and protein kinase C activation fail to initiate a T cell proliferation in the absence of a costimulatory signal.  J. Immunol.. 1990;;  144 3701-9
• 19 Kilpatric  D C,, Mc. Currach  P M.. The wheat germ agglutinating is mitogenic, nonmitogenic and antimitogenic for human lymphocytes.  Scan. J. Immunol.. 1987;;  25 343-8
• 20 Kilpatric  D C,, Pusztai  A,, Grant  G,, Graham  C,, Ewans  S WB.. Tomato lectin resists digestion in the mammalian alimentary canal and binds to intestinal villi without deleterious effects.  FEBS Lett.. 1985;;  185 299-305