Planta Med 2001; 67(1): 70-73
DOI: 10.1055/s-2001-10622
Original Paper
Phytochemical Analysis
© Georg Thieme Verlag Stuttgart · New York

Quantitative Determination of Secoiridoid Glucosides in In Vitro Propagated Plants of Gentiana davidii var. formosana by High Performance Liquid Chromatography

Fu-Shin Chueh1 , Chung-Chuan Chen1 , Abhay P. Sagare2 , Hsin-Sheng Tsay2,*
  • 1 Institute of Chinese Pharmaceutical Science, China Medical College, Taichung, Taiwan
  • 2 Department of Agronomy, Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan
Further Information

Publication History

February 15, 2000

May 13, 2000

Publication Date:
31 December 2001 (online)

Abstract

A simple protocol for in vitro mass propagation of Gentiana davidii var. formosana (Gentianaceae) has been developed. Multiple shoot development was achieved by culturing the stem node explants on Murashige and Skoog (MS) medium supplemented with 4.44 μM N 6-benzyladenine (BA). The shoots were multiplied by subculturing on MS medium supplemented with 1.07 - 10.74 μM α-naphthaleneacetic acid (NAA) and 8.88 μM BA. Shoots were rooted on MS basal medium supplemented with various auxins. Shoots rooted on growth regulator-free medium were transferred to peat moss : vermiculite mixture and acclimatized in the growth chamber. The contents of gentiopicroside and swertiamarin, the two important secoiridoid glucosides, in different plant material were determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of gentiopicroside and swertiamarin in the aerial and underground parts of G. davidii var. formosana was higher than the marketed crude drug (underground parts of G. scabra) and varied with the age of the plant.

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Dr. Hsin-Sheng Tsay

Department of Agronomy

Taiwan Agricultural Research Institute

Wu-feng

Taichung 41301

Taiwan

Republic of China

Email: hstsay@wufeng.tari.gov.tw

Fax: +886-4-3338162