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DOI: 10.1055/s-2001-10634
© Georg Thieme Verlag Stuttgart · New York
Inhibitory Effect of Tetrandrine on Dopamine Biosynthesis and Tyrosine Hydroxylase in PC12 Cells
Publication History
February 29, 2000
July 16, 2000
Publication Date:
31 December 2001 (online)
![](https://www.thieme-connect.de/media/plantamedica/200101/lookinside/thumbnails/10.1055-s-2001-10634-1.jpg)
Abstract
The effect of tetrandrine, a bis-benzylisoquinoline alkaloid, on dopamine biosynthesis in PC12 cells was investigated. Tetrandrine at a concentration of 3.0 μM decreased dopamine content by 59.4 % (IC50 = 2.4 μM) and intracellular tyrosine hydroxylase (TH) activity was inhibited by the treatment of tetrandrine (49.8 % inhibition at 3.0 μM) compared with control. We next examined the effects of tetrandrine on the kinetics of PC12 TH. The PC12 TH was obtained from PC12 cells with minor purification. Tetrandrine inhibited the PC12 TH activity by 40.6 % at a concentration of 45 μM and exhibited noncompetitive inhibition on the enzyme using L-tyrosine as a substrate (Ki = 60.8 μM). These results suggest that the inhibition of TH activity by tetrandrine may partially contribute to the decrease in dopamine biosynthesis in PC12 cells.
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Lian-Hua Fang
Department of Pharmacology
College of Medicine
Chungbuk National University
San 48, Kaeshin-Dong
Cheongju 361-763
South Korea
Email: fanglh@medicine.chungbuk.ac.kr
Fax: (+82) 43-272-1603