Planta Med 2001; 67(2): 150-152
DOI: 10.1055/s-2001-11519
Original Paper

© Georg Thieme Verlag Stuttgart · New York

Growth and Production of Camptothecin by Cell Suspension Cultures of Nothapodytes foetida

Devanand P. Fulzele*, Ramesh K. Satdive, B. B. Pol
  • Plant Biotechnology and Secondary Products Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India
Further Information

Publication History

March 23, 2000

October 8, 2000

Publication Date:
31 December 2001 (online)

Abstract

Callus cultures were initiated from stem parts of Nothapodytes foetida on Murashige and Skoog's medium supplemented with different growth regulators. Suspension cultures were established and the cell biomass was higher in the presence of NAA in comparison with 2,4-D. Culture medium supplemented with NAA (10.74 μM) and BA (2.22 μM) attained 31.3 g/l DW during 20 days of cultivation in shake flasks. In the presence of NAA, maximum concentrations of camptothecin (0.035 mg/ml) and 9-methoxycamptothecin (0.026 mg/ml) were found in the medium. Alkaloid production was reduced in presence of 2,4-D in the culture medium. Cells contained trace amount of alkaloids. Alkaloids were detected and identified by means of TLC and HPLC.

References

  • 1 Aiyama R, Nagai H, Kokata K, Shinonara C, Sawada S. A camptothecin derivatives from Nothapodytes foetida .  Phytochemistry. 1988;  27 3666-4
  • 2 Priel E, Showalter S D, Blair D G. Inhibition of human immunodificiency virus (HIV-1) replication in vitro by non-cytotoxic doses of camptothecin, a topoisomerase I inhibitor.  AIDS Research and Human Retroviruses. 1991;  7 65-72
  • 3 Takeuchi A, Dohashi K, Fujimoto S, Tanaka K, Suzuki M, Terashima Y et al. A late phase II study of CPT-II in uterine cervical cancer and ovarian cancer.  Jap. J. Cancer Chemotherapy. 1991;  18 1681
  • 4 Potmesil M. Camptothecin from bench research to hospital wards.  Cancer Research. 1994;  54 1431-9
  • 5 Bodley A F, Cumming J N, Shapiro T A. Effects of camptothecin, 1 toppisomerase I inhibitor, on Plasmodium falciparum .  Biochem. Pharma.. 1998;  55 709-11
  • 6 Sakato K, Tanaka H, Mukai N, Misawa M. Isolation and identification of camptothecin from cells of Camptotheca acuminata suspension cultures.  Agri. Biol. Chem. 1974;  38 217-8
  • 7 Van-Hengel A J, Harkes M P, Wichers H J, Hesselink P GM, Buitelaar R M. Characterization of callus formation and camptothecin production by cell lines of Camptotheca acuminata .  Plant Cell, Tissue and Organ Culture. 1992;  28 11-8
  • 8 Wiedenfeld H, Furmanowa M, Roeder E, Guzewska J, Gustowski W. Camptothecin and 10-hydroxycamptothecin in callus and plantlets of Camptotheca acuminata .  Plant Cell, Tissue and Organ Culture. 1997;  49 213-8
  • 9 Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures.  Physiol. Plant. 1962;  15 473-9
  • 10 Fulzele D P, Heble M R. Large scale cultivation of Catharanthus roseus cells: Production of ajmalicine in a 20 l air-lift bioreactor.  J. Biotechnology. 1994;  35 1-7
  • 11 Fulzele D P, Heble M R, Rao P S. Production of terpenoid from Artemisia annua L. Plantlet culture in bioreactor.  J. Biotechnology. 1995;  40 139-43
  • 12 Zenk M H, El-Shagi H, Schulte U. Anthraquinone production by cell suspension culture of Morinda citrifolia .  Planta Medica Suppl.. 1975;  79 101
  • 13 Ohta S, Yatazawa M. Growth and alkaloid production in callus tissue of Rauwolfia serpentina .  Biol. Chem. 1979;  43 2297-303
  • 14 Van-Der-Plas L HW, Eijkelboom C, Hageldoorn M JM. Relation between primary and secondary metabolism in plant cell suspensions.  Plant Cell, Tissue and Organ Culture. 1995;  43 111-6
  • 15 Nahalka J, Blaarik P, Gemeiner P, Matusova F, Part Iova. Production of plumbagin by cell suspension cultures of Drosophyllum lusitanicum, Link.  J. Biotechnology. 1996;  49 153-61
  • 16 Whitmer S, Verpoorte R, Canel C. Influence of auxins on alkaloid accumulation by a transgenic cell line of Catharanthus roseus .  Plant Cell, Tissue and Organ Culture. 1998;  53 135-41

Dr. Devanand P. Fulzele

Plant Biotechnology and Secondary Products Section

Nuclear Agriculture and Biotechnology Division

Bhabha Atomic Research Centre, Trombay

Mumbai-440 085

India

Email: dfulzele@apsara.barc.ernet.in

Fax: +91 22 5505151