Identification of Volatile Constituents of Tambourissa leptophylla

Abstract

The volatile fraction of Tambourissa leptophylla fruit skin was extracted by petrol ether, purified by adsorption chromatography (LPC) and analysed by gas chromatographic-spectroscopic methods. 27 non-oxygenated terpene hydrocarbons and 10 oxygenated derivatives were identified. The most abundant components were: limonene (24.0 %), cis-α-bergamotene (23.2 %), Δ-3-carene (8.2 %), α-curcumene (6.0 %), trans-α-bergamotene (5.1 %), α-copaene (4.1 %), α-pinene (4.0 %), p-cymene (4.0 %) and bicyclogermacrene (3.3 %). The crude volatile fraction was tested in vitro against Cladosporium cucumerinum in direct bioautography on TLC plates on the basis of the antifungal use of fruit skin. Activity of petrol ether extract against this micro-organism was demonstrated.

References

• 1 Contribution aux étude ethnobotaniques et florístique aux Comores. Agence de Coopération Culturelle et Technique Editor 1982: 99
• 2 Forgacs P, Buffard G, Desconclois J F, Jehanno A, Provost J. Études phytochimiques et activités biologiques des plantes endémiques de l'Ile de la Réunion et de l'Ile Maurice.  Plantes Medicinales et Phytotherapie. 1981;  15 80-91
• 3 Rahalison L, Hamburger M, Monod M, Frenk E, Hostettmann K. Antifungal tests in phytochemical investigations: comparison of bioautographic methods using phytopathogenic and human pathogenic fungi.  Planta Medica. 1994;  60 41-4
• 4 Rahalison L, Hamburger M, Hostettmann K, Monod M, Frenk E, Gupta M P, Santana A I, Correra A MD, Gonzalez A G. Screening for antifungal activity of Panamanian plants.  International Journal of Pharmacognosy. 1993;  31 68-76