Horm Metab Res 2001; 33(1): 7-9
DOI: 10.1055/s-2001-12618
Original Basic
© Georg Thieme Verlag Stuttgart · New York

Evidence of Sex Hormone Binding Globulin Binding Sites in the Medial Preoptic Area and Hypothalamus

J. D. Caldwell
  • Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND, USA
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Publikationsverlauf

Publikationsdatum:
31. Dezember 2001 (online)

We have demonstrated a high density of both radiolabeled progesterone and estradiol conjugated to bovine serum albumin binding sites in the medial preoptic area and hypothalamus. Infusions of sex hormone binding globulin into the medial preoptic area of rats increased their female sexual receptivity similarly to the effect of estradiol conjugated to bovine serum albumin, suggesting sex hormone binding globulin acts at binding sites for estradiol conjugated to bovine serum albumin. In this study sex hormone binding globulin was used to displace radiolabeled progesterone conjugated to bovine serum albumin from plasma membrane fractions from the medial preoptic area-anterior hypothalamus and medial basal hypothalamus of ovariectomized rats injected with either 5 μg estradiol benzoate or sesame oil vehicle. We found that sex hormone binding displaced radiolabeled progesterone conjugated to bovine serum albumin in both areas and that in vivo estradiol treatment greatly increased the relative displacement by sex hormone binding globulin in the medial preoptic area-anterior hypothalamus. We interpret these data as indicating the presence of sex hormone binding globulin receptors in brain plasma membranes and further suggest that endogenous steroid conditions may alter these receptors.

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J. D. Caldwell,Ph.D. 

Department of Biomedical Sciences
University of Illinois College of Medicine

1601Parkview Avenue
Rockford, IL 61107-1897
USA


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