Characterization of Root Exudates at Different Growth Stages of Ten Rice (Oryza sativa L.) Cultivars

M. S. Aulakh; R. Wassmann; C. Bueno; J. Kreuzwieser; H. Rennenberg

doi:10.1055/s-2001-12905

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2001; 3(2): 139-148
DOI: 10.1055/s-2001-12905

Original Paper

Georg Thieme Verlag Stuttgart ·New York

Characterization of Root Exudates at Different Growth Stages of Ten Rice (Oryza sativa L.) Cultivars

M. S. Aulakh ^1,2 , R. Wassmann ^1,3 , C. Bueno ¹ , J. Kreuzwieser ⁴ , H. Rennenberg ^3,4

¹ International Rice Research Institute, Soil and Water Sciences Division, Makati City, Philippines
² Present address: Department of Soils, Punjab Agricultural University, Ludhiana , Punjab, India
³ Fraunhofer Institute for Atmospheric Environmental Research, Garmisch-Partenkirchen, Germany
⁴ 4Institute for Forest Botany and Tree Physiology, University of Freiburg, Freiburg, Germany

Abstract

Plant root exudates play important roles in the rhizosphere. We tested three media (nutrient solution, deionized water and CaSO₄ solution) for three periods of time (2, 4 and 6 h) for collecting root exudates of soil-grown rice plants. Nutrient culture solution created complications in the analyses of exudates for total organic C (TOC) by the wet digestion method and of organic acids by HPLC due to the interference by its components. Deionized water excluded such interference in analytical analyses but affected the turgor of root cells; roots of four widely different rice cultivars excreted 20 to 60 % more TOC in deionized water than in 0.01 M CaSO₄. Furthermore, the proportion of carbohydrates in TOC was also enhanced. Calcium sulfate solution maintained the osmotic environment for root cells and did not interfere in analytical procedures. Collection for 2 h avoided under-estimation of TOC and its components exuded by rice roots, which occurred during prolonged exposure. By placing plants in 0.01 M CaSO₄ for 2 h, root exudates of soil-grown traditional, tall rice cultivars (Dular, B40 and Intan), high-yielding dwarf cultivars (IR72, IR52, IR64 and PSBRc 20), new plant type cultivars (IR65598 and IR65600) and a hybrid (Magat) were collected at seedling, panicle initiation, flowering and maturity and characterized for TOC and organic acids. The exudation rates were, in general, lowest at seedling stage, increased until flowering but decreased at maturity. Among organic acids, malic acid showed the highest concentration followed by tartaric, succinic, citric and lactic acids. With advancing plant growth, exudation of organic acids substituted exudation of sugars. Root and shoot biomass were positively correlated with carbon exudation suggesting that it is driven by plant biomass. As root exudates provide substrates for methanogenesis in rice fields, large variations in root exudation by cultivars and at different growth stages could greatly influence CH₄ emissions. Therefore, the use of high-yielding cultivars with lowest root excretions, for example IR65598 and IR65600, would mediate low exudate-induced CH₄ production. The screening of exciting rice cultivars and breeding of new cultivars with low exudation rates could offer an important option for mitigation of CH₄ emission from rice agriculture to the atmosphere.

Key words

Root exudates - organic carbon - organic acids - carbohydrates - rice cultivars - growth stages - methane production

Full Text

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H. Rennenberg

Institute for Forest Botany and Tree Physiology
Albert-Ludwigs University

79085 Freiburg
Germany

Email: here@uni-freiburg.de

Section Editor: U. Lüttge