Planta Med 2001; 67(4): 379-381
DOI: 10.1055/s-2001-14310
Letter

© Georg Thieme Verlag Stuttgart · New York

Differentiation of Lycium barbarum from its Related Lycium Species using Random Amplified Polymorphic DNA

Kalin Y. B. Zhang1 , H. W. Leung1 , H. W. Yeung1 , Ricky N. S. Wong1, 2,*
  • 1 Institute for the Advancement of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
  • 2 Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
Weitere Informationen

Publikationsverlauf

June 8, 2000

July 29, 2000

Publikationsdatum:
31. Dezember 2001 (online)

Abstract

The RAPD (random amplified polymorphic DNA) technique was applied for the first time to distinguish Lycium barbarum from other closely related species of the same genus. In this study, eight samples were collected, including five species, two varieties and one cultivated variety. A total of fifty arbitrary primers were used in the RAPD analysis. Distinctive DNA fingerprints corresponding to different Lycium species were successfully obtained from ten primers. Similarity index (S.I.) analysis revealed that the values are higher between intraspecies than interspecies. These results confirmed that the RAPD technique can be employed for distinguishing closely related species of Lycium.

References

  • 1 Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers.  Nucleic Acids Research. 1990;  18 7213-8
  • 2 Williams J GK, Kubelik A R, Livak K J, Rafalski J A, Tingey S V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.  Nucl. Nucleic Acids Research. 1990;  18 6531-5
  • 3 Cheung K S, Kwan H S, But P PH, Shaw P C. Pharmacognostial identification of American and Oriental ginseng roots by genomic fingerprinting using arbitrarily primed polymerase chain reaction (AP-PCR).  Journal of Ethnopharmacol. 1994;  42 67-9
  • 4 Yamazaki M, Sato A, Shimomura K, Saito K, Murakoshi I. Genetic relationships among Glycyrrhiza plants determined by RAPD and RFLP analyses.  Biological pharmaceutical bulletin. 1994;  17 1529-31
  • 5 Kohjyouma M, Iida O, Yoshida N, Hatakeyama Y, Satake M, Sekita S Kohda. Random amplified polymorphic DNA analysis of Angelica acutilova and its varieties.  Natural Medicines. 1998;  52 130-4
  • 6 Cheng K T, Su C H, Chang H C, Huang J Y. Differentiation of genuines and counterfeits of Cordyceps species using random amplified polymorphic DNA.  Planta Medica. 1998;  64 451-3
  • 7 Zhang Y B, Ngan F N, Wang Z T, Ng T B, But P PH, Shaw P C, Wang J. Random primed polymerase chain reaction differentiates Codonopsis pilosula from different localities.  Planta Medica. 1999;  65 157-60
  • 8 Cheng K T, Tsay H S, Chen C F, Chou T W. Determination of the components in a Chinese prescription, Yu-Ping-Feng San, by RAPD analysis.  Planta Medica. 1998;  64 563-5
  • 9 Draper J, Scott R. Plant genetic transformation and gene expression. In: Draper J, Scott R, Armitage P, Walden R, editors A Laboratory manual. London; Blackwell Scientific Publishing Co. 1988: 199-236
  • 10 Nadeau J H, Bedigian H G, Bouchard G, Denial T, Kosowsky M, Norberg R, Pugh S, Sargeant E, Turner R, Paigen B. Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence.  Mammalian Genome. 1992;  3 55-64

Dr. Ricky N. S. Wong

Department of Biology

Hong Kong Baptist University

Kowloon Tong

Hong Kong

China

eMail: rnswong@hkbu.edu.hk

Fax: 852-2339-5995