Characters of nrDNA ITS Region Sequences of Fruits of Alpinia galanga and Their Adulterants

Abstract

The fruits of Alpinia galanga (L.) Sw. are used as a traditional Chinese medicine; but the dry fruits of A. conchigera, A. suishaensis, A. maclurei and A. polyantha are also used as the medicine in local areas. Because dry fruits of these related plants are similar to those of Alpinia galanga (L.) Sw. in odor, morphological characters and chemical components, and even anatomical characters, it is difficult to identify the medicine. Nuclear ribosomal DNA internal transcribed spacer (ITS) regions of the five taxa were directly sequenced using an automated sequencer. Sequence analysis showed that the ITS 1 ranges from 177 to 178 base pairs (bp), and the ITS 2 from 225 to 234 bp. The size of the 5.8S coding region is 164 bp for all species. Also, the pairwise sequence divergence is higher and some molecular markers were determined. According to these molecular markers, Alpinia galanga (L.) Sw. and the related species can easily be distinguished from each other. Therefore, evidence from nrDNA ITS sequence variation can identify the medicine at the DNA level.

References

• 1 Wu Z Y, Zhou T Y, Xiao P G. Xin Hua Ben Cao Gang Yao, vol. 1. Shanghai Science & Technology Press 1988: 538
• 2 Zhao Z L, Li B, Dong H, Qin M J, Mei Q C, Xu L S et al. A survey of galangal (Alpinia Roxb.) resources of China.  Chinese Traditional and Herbal Drugs. 1998;  29 621-5
• 3 Wen S K, Wei J F. Identification of Hong Dou Kou and two adulterants.  Bulletin of Chinese Materia Medica. 1987;  12 649-50
• 4 Chen Y H, Tong Y Y, Duan H, Shang J H. Studies on medicinal plants of Zingiberaceae from China: III. Identification of origin and medicinal materials of Hong Dou Kou.  Journal of Chinese Medicinal Materials. 1984;  7 (2) 24-31
• 5 Wu T L, Wu Q G, Chen Z Y. Proceedings of the second symposium on the family Zingiberaceae. Zhongshan University Press 1996: 82-90
• 6 Suh Y, Thien L B, Reeve H E, Zimmer E A. Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA in Winteraceae.  American Journal of Botany. 1993;  80 (9) 1042-55
• 7 Fu R Z, Wang J, Zhang Y B, Wang Z T, But P PH, Li N et al. Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism.  Planta Medica. 1999;  65 648-50
• 8 Zhang Y B, Ngan F N, Wang Z T, Ng T B, But P PH, Shaw P C et al. Random primed polymerase chain reaction differentiates Codonopsis pilosula from different localities.  Planta Medica. 1999;  65 157-60
• 9 Cai Z H, Li P, Dong T TX, Tsim K WK. Molecular diversity of 5S-rRNA spacer domain in Fritillaria species revealed by PCR analysis.  Planta Medica. 1999;  65 360-4
• 10 Alice L A, Campbell C S. Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences.  American Journal of Botany. 1999;  86 (1) 81-97
• 11 Steane D A, Scotland R W, Mabberley D J, Olmstead R G. Molecular systematics of Clerodendrum (Lamiaceae): ITS sequences and total evidence.  American Journal of Botany. 1999;  86 (1) 98-107
• 12 Baldwin B G, Sanderson M J, Porter J M, Wojciechowski M F, Campbell C S, Donoghue M J. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny.  Annals of the Missouri Botanical Garden. 1995;  82 247-77
• 13 Zhao Z L, Dong H, Xu L S. A study on the systematic status of Alpinia calcarata Rosc.  Chinese Wild Plant Resources. 1999;  18 (5) 4-5
• 14 Takaiwa F, Oono K, Sugiura M. Nucleotide sequence of the 17S-25S spacer region from rice rDNA.  Plant Molecular Biology. 1985;  4 355-64
• 15 Ge S, Schaal B A, Hong D Y. Reevaluation of the status of A. lobophylla based on ITS sequence, with reference to the utility of ITS sequence in Adenophora .  Acta Phytotaxonomica Sinica. 1997;  35 385-95