Biodisposition of FITC-Labeled Aloemannan in Mice

Abstract

Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine into smaller molecules that were effectively absorbed in mice. The homogenate from the other tissues did not affect the metabolism of F-AM. The degraded product (1) of F-AM after incubation with 10 % feces homogenate for 24 h was chromatographed on a highly porous polymer and a Sephadex LH-20 column to provide an FITC-degraded fraction (2), which was shown to have a molecular weight of 800 D on Sephadex G-25 gel permeation. Metabolite 2 was examined by physicochemical methods and shown to be a mixture of FITC-hexose and -2 hexose on FAB-MS. An FITC-degraded fraction (3) with a molecular weight of 3 KD was obtained by 6-h incubation with 10 % feces homogenate on Sephadex G-25 column chromatography and was shown to be a mixture of FITC-9 and 12 × hexose on TOF-MS.

Key words

Aloemannan - Aloe vera - Asphodelaceae - fluoresceinyl isothiocyanate aloemannan - metabolism - degraded fluoresceinyl thiocyanate aloemannan - fast atom bombartment mass spectrometry - matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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