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DOI: 10.1055/s-2001-14328
© Georg Thieme Verlag Stuttgart · New York
Effects of Baicalein Isolated from Scutellaria baicalensis Radix on Adhesion Molecule Expression Induced by Thrombin and Thrombin Receptor Agonist Peptide in Cultured Human Umbilical Vein Endothelial Cells
Publikationsverlauf
May 5, 2000
October 10, 2000
Publikationsdatum:
31. Dezember 2001 (online)
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Abstract
We examined the effects of various flavonoids isolated from the roots of Scutellaria baicalensis Georgi on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide (SFLLRNPNDKYEPF, TRAP) in cultured human umbilical vein endothelial cells. Thrombin and thrombin receptor agonist peptide induced endothelial leukocyte adhesion molecule-1 (ELAM-1) expression. Intercellular adhesion molecule-1 (ICAM-1) expression was also induced by thrombin, but not by TRAP. Baicalein isolated from Scutellariae Radix inhibited ELAM-1 expression induced by thrombin and thrombin receptor agonist peptide dose-dependently, with 50 % inhibitory concentrations (IC50) of 5.53 ± 1.68 μM and 2.44 ± 1.08 μM, respectively. Furthermore, baicalein inhibited thrombin-induced ICAM-1 expression with an IC50 of 9.67 ± 1.28 μM. In addition, baicalein inhibited the expressions of ELAM-1 and ICAM-1 stimulated by protein kinase C (PKC) activator phorbol myristate acetate (PMA).
Key words
Baicalein - Scutellaria baicalensis - Labiatae - adhesion molecules - thrombin - thrombin receptor agonist peptide - human umbilical vein endothelial cells
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Dr. Yoshiyuki Kimura
Second Department of Meical Biochemistry
School of Medicine
Ehime University
Shigenobu-cho
Onsen-gun
Ehime 791-0295
Japan
eMail: yokim@m.ehime-u.ac.jp
Fax: +81-89-960-5256
Telefon: Tel.: +81-89-960-5254