Effects of Baicalein Isolated from Scutellaria baicalensis Radix on Adhesion Molecule Expression Induced by Thrombin and Thrombin Receptor Agonist Peptide in Cultured Human Umbilical Vein Endothelial Cells

Abstract

We examined the effects of various flavonoids isolated from the roots of Scutellaria baicalensis Georgi on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide (SFLLRNPNDKYEPF, TRAP) in cultured human umbilical vein endothelial cells. Thrombin and thrombin receptor agonist peptide induced endothelial leukocyte adhesion molecule-1 (ELAM-1) expression. Intercellular adhesion molecule-1 (ICAM-1) expression was also induced by thrombin, but not by TRAP. Baicalein isolated from Scutellariae Radix inhibited ELAM-1 expression induced by thrombin and thrombin receptor agonist peptide dose-dependently, with 50 % inhibitory concentrations (IC₅₀) of 5.53 ± 1.68 μM and 2.44 ± 1.08 μM, respectively. Furthermore, baicalein inhibited thrombin-induced ICAM-1 expression with an IC₅₀ of 9.67 ± 1.28 μM. In addition, baicalein inhibited the expressions of ELAM-1 and ICAM-1 stimulated by protein kinase C (PKC) activator phorbol myristate acetate (PMA).

Key words

Baicalein - Scutellaria baicalensis - Labiatae - adhesion molecules - thrombin - thrombin receptor agonist peptide - human umbilical vein endothelial cells

References

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