Development and Application of Expressed Protein Ligation

Tom W. Muir

doi:10.1055/s-2001-14577

Synlett 2001; 2001(6): 0733-0740
DOI: 10.1055/s-2001-14577

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Development and Application of Expressed Protein Ligation

Tom W. Muir^*

^*Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Ave., New York, NY 10021, USA; Fax 212 327 7338; E-mail: muirt@rockvax.rockefeller.edu

Abstract

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Expressed protein ligation (EPL) is a protein semi-synthesis approach that allows synthetic and recombinant polypeptides to be chemoselectively and regioselectively joined together via a peptide bond. The approach makes the primary structure of proteins accessible to the tools of synthetic organic chemistry and thus provides expanded opportunities in the area of protein engineering. The development and application of this process will be discussed.

chemical ligation - inteins - semi-synthesis

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