Planta Med 2001; 67(5): 451-455
DOI: 10.1055/s-2001-15801
Original Paper
Analysis
© Georg Thieme Verlag Stuttgart · New York

Supercritical Fluid Extraction and Analysis of Compounds from Clivia miniata for Uterotonic Activity

Vikash Sewram1,*, Mark W. Raynor1, 2 , Dulcie A. Mulholland1 , Deshandra M. Raidoo3
  • 1 Department of Chemistry and Aplied Chemistry, University of Natal, Durban, South Africa
  • 2 Matheson Gas Products, Advanced Technology Centre, Longmont, Colorado, USA
  • 3 Department of Clinical & Experimental Pharmacology, University of Natal, Medical School, Congella, South Africa
Weitere Informationen

Publikationsverlauf

July 20, 2000

August 9, 2000

Publikationsdatum:
31. Dezember 2001 (online)

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Abstract

In this descriptive study, the superciritical fluid extract of the roots of Clivia miniata L. was tested for uterotonic activity using guinea pig uterine smooth muscle in vitro. Extraction was performed with water modified supercritical carbon dioxide at 400 atm and 80 °C. The uterine contractions induced by this extract were compared to those induced by the aqueous extract and found to be active at lower doses. The active compounds were isolated and the structures elucidated by spectroscopic and chromatographic techniques. Both linoleic acid and 5-hydroxymethyl-2-furancarboxaldehyde isolated from the extract were found to induce muscle contractions individually. The pharmacological mode of action of 5-hydroxymethyl-2-furancarboxaldehyde was assessed using two receptor agonists and antagonists. This compound was found to mediate its effect through cholinergic receptors.

References

Dr. Vikash Sewram

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Medical Research Council

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