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DOI: 10.1055/s-2001-15818
© Georg Thieme Verlag Stuttgart · New York
Authentication of Medicinal Dendrobium Species by the Internal Transcribed Spacer of Ribosomal DNA
Publikationsverlauf
July 20, 2000
November 11, 2000
Publikationsdatum:
31. Dezember 2001 (online)
![](https://www.thieme-connect.de/media/plantamedica/200105/lookinside/thumbnails/10.1055-s-2001-15818-1.jpg)
Abstract
Herba Dendrobii (Shihu) is a commonly used Chinese medicine derived from the stem of several orchid species belonging to the genus Dendrobium. It is rather expensive and adulteration is frequent. Proper authentication of the medicinal species is necessary to protect consumers and support conservation measures. DNA sequences of the internal transcribed spacer 2 (ITS 2) of 16 Dendrobium species were shown to be significantly different from one another by an average of 12.4 % and from non-orchids and Pholidota (an adulterant of Shihu) by 29.8 % and 18.8 %, respectively. The intra-specific variation among the Dendrobium species studied was only about 1 %. Therefore, ITS 2 regions could be adopted as a molecular marker for differentiating medicinal Dendrobium species from one another and also from non-orchids and adulterants.
Key words
Dendrobium - Orchidaceae - DNA sequence - orchid - endangered species
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Prof Paul Pui-Hay But
Department of Biology and Institute of Chinese Medicine
The Chinese University of Hong Kong
Shatin, N.T
Hong Kong
eMail: paulbut@cuhk.edu.hk
Fax: (852) 2603-5248
Telefon: Tel: (852) 2609-6140