Abstract
Micromorphological analysis reveals that A. jahandiezii, which occurs as a local endemic taxon in the Grand Canyon du Verdon in southern France, shows substantial differences to A. bourgaei, from the eastern Mediterranean region, in the surface structure of perispores, sporangial indehiscence and the size of stomatal subsidiary cells. Nucleotide sequences and genetic distances (0.9 % nucleotide substitutions) of the rbc L gene support the view that both taxa are related but distinct sibling species. Both species form a monophyletic group together with Asplenium fontanum and A. majoricum.
Key words
Asplenium
-
rbc L sequences - sibling species - molecular phylogeny
References
01
Brownsey, P. J..
(1977);
An example of sporangial indehiscence in the Filicopsida.
Evolution.
31
294-301
02
Cotthem, W. van.
(1970);
A classification of stomata types.
Bot. J. Linn. Soc..
63
235-246
03 Cotthem, W. van. (1973) Stomatal types and systematics. The phylogeny and classification of the ferns. Jermy, A. C. et al., eds. London; Academic Press pp. 59-71
04 Crabbe, J. A.. (1993) 1. Asplenium.
. Flora Europaea, Vol. 1, Psilotaceae to Platanceae, 2nd ed. Tutin, T. G. et al., eds. Cambridge; University Press pp. 18-23
05
Doyle, J. J., and Doyle, J. L..
(1990);
Isolation of plant DNA from fresh tissue.
Focus.
12
13-15
06
Greuter, W. et al..
(1983);
Asplenium bourgaei, a new addition to the flora of Europe.
Fern. Gaz..
12
271-274
07 Kramer, K. U., and Viane, R.. (1990) Aspleniaceae. The families and genera of vascular plants, Vol. I, Pteridophytes and Gymnosperms, (Kramer, K. U. and Green P. S., Vol. eds). Kubitzki, K., ed. Berlin, Heidelberg; Springer pp. 52-57
08 Kumar, S.,, Tamura, K.,, and Nei, M.. (1993) MEGA-Molecular Evolutionary Genetics Analysis, Version 1.0. Pennsylvania State University
09
Meyer, D. E..
(1962);
Über neue und seltene Asplenien Europas.
Ber. Dtsch. Bot. Ges..
75
24-34
10
Rasbach, H.,, Reichstein, T.,, and Viane, R..
(1994);
Asplenium chiuahuense (Aspleniaceae, Pteridophyta), an allohexaploid species and the description of a simplified hybridisation technique.
Amer. Fern J..
84
11-40
11 Swofford, D. L.. (1998) PAUP: Phylogenetic analysis using parsimony, version 4.0b4a. Sunderland, Mass.; Sinauer Associates, Inc. Publ
M. Wink
Institut für Pharmazeutische Biologie
INF 364 69120 Heidelberg
Email: wink@uni-hd.de
Section Editor: M. Hasebe