Planta Med 2001; 67(8): 714-717
DOI: 10.1055/s-2001-18353
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Procyanidins from Vitis vinifera Seeds Inhibit the Respiratory Burst of Activated Human Neutrophils and Lysosomal Enzyme Release

Marina Carini*, Rita Stefani, Giancarlo Aldini, Marica Ozioli, Roberto Maffei Facino
  • Istituto Chimico Farmaceutico Tossicologico, University of Milan, Italy
Further Information

Publication History

October 31, 2000

February 15, 2001

Publication Date:
09 November 2001 (online)

Abstract

The inhibitory properties of procyanidins (a standardized oligomeric catechin fraction) from Vitis vinifera L. seeds on the respiratory burst and on the release of granule components myeloperoxidase, β-glucuronidase and elastase were studied in activated human neutrophils. Procyanidins strongly inhibit superoxide generation with an IC50 of 7.2 μM, through a direct scavenging of superoxide and prevent the release from calcium ionophore activated neutrophils of β-glucuronidase (IC50 = 13.9 μM), myeloperoxidase (IC50 = 7.2 μM) and elastase (IC50 = 5.4 μM). In addition they dose-dependently inhibit the activity of myeloperoxidase released from calcium ionophore-stimulated cells with an IC50 value of 2 μM. The monomeric constitutive unit (+)-catechin was far less active than procyanidins in all the models tested. These results evidence that procyanidins efficiently restrain the inflammatory response of activated neutrophils in vitro and whenever absorbed in vivo can prevent their oxidative discharge at the site(s) of their adhesion.

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Prof. Dr. Marina Carini

Istituto Chimico Farmaceutico Tossicologico

Viale Abruzzi 42

20131 Milan

Italy

Email: Marina.Carini@unimi.it

Fax: +39-02-58357565