Planta Med 2001; 67(9): 815-819
DOI: 10.1055/s-2001-18855
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Antigenotoxicity of trans-Dehydrocrotonin, a Clerodane Diterpene from Croton cajucara

Aniele R. Agner1 , Maria  A. M. Maciel2 , Angelo C. Pinto2 , Ilce M. S. Cólus1
  • 1 Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina (Pr), Brazil
  • 2 Instituto de Química - Centro de Tecnologia Bloco A, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
Weitere Informationen

Publikationsverlauf

December 8, 2000

March 31, 2001

Publikationsdatum:
06. Dezember 2001 (online)

Abstract

The antigenotoxic action of three doses of trans-dehydrocrotonin (t-DCTN), the active ingredient obtained from the bark extract of Croton cajucara, a plant native to the Amazon, was determined in Swiss mice in vivo. Mice were submitted to acute intraperitoneal and gavage treatments, then their bone marrow cells were subsequently analyzed by micronucleus (MN) and chromosome aberration (CA) assays. Comparisons were performed between the three doses of t-DCTN and the negative-control group. Statistical analysis indicated that doses of 50 and 75 % of the LD50, via intraperitoneal treatment or gavage injection, were antimutagenic with regard to cyclophosphamide. However, the dose of 25 % of the LD50 was only antimutagenic when administered by gavage. Based on these observations, it can be suggested that gavage is the most effective method of administering t-DCTN. In addition, t-DCTN showed no cytotoxic effects in the bone marrow cells regardless of the route of exposure.

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Dr. Ilce M. S. Cólus

Departamento de Biologia Geral - CCB - UEL - Pr - Brazil

Centro de Ciências Biológicas

Universidade Estadual de Londrina

Campus Universitário

CEP 86051 - 970 Londrina (Pr)

Brazil

eMail: colus@sercomtel.com.br

Fax: (43) 371-4207

Telefon: Tel.: (43) 371-4417