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Horm Metab Res 2001; 33(12): 713-720
DOI: 10.1055/s-2001-19133
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Microvascular Permeability is Increased in Both Types of Diabetes and Correlates Differentially with Serum Levels of Insulin-Like Growth Factor I (IGF-I) and Vascular Endothelial Growth Factor (VEGF)

F. Brausewetter 1 , P. M. Jehle 1 , M. F. Jung 1 , B. O. Boehm 2 , J. Brueckel 2 , V. Hombach 1 , H. H. Osterhues 1
  • 1 Department of Medicine II, University of Ulm, Germany
  • 2 Department of Medicine I, University of Ulm, Germany
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Publication History

Publication Date:
18 December 2001 (online)

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Vascular endothelial growth factor (VEGF) and insulin-like growth factor-I (IGF-I) both play a pivotal role in diabetic microangiopathy. This study assessed the relationship between capillary permeability as a marker of endothelial dysfunction and serum VEGF and IGF-I levels in normotensive diabetics. Subjects were 10 Type 1 (6/4, male/female, age: 30 [mean] ± 5 [SD] years, HbA1c: 7.5 ± 1.1 %), 13 Type 2 diabetics (9/4, m/f; 63 ± 7 years, 8.3 ± 1.8 %), and 24 age- and sex-matched control subjects. We determined nailfold capillary permeability by intravital fluorescence videomicroscopy after intravenous injection of sodium-fluorescein. Serum VEGF, free and total IGF-I, IGF binding protein (IGFBP)-1, IGFBP-3, and insulin levels were measured by specific immunoassays. Capillary permeability was increased in both types of diabetes patients compared to age- and sex-matched controls. In Type 1 diabetics, fluorescence light intensities increased over time, reaching significance 30 minutes after dye injection. Type 2 diabetics already revealed an early onset of elevated fluorescence light intensities after one minute. Capillary permeability showed a significant positive correlation with VEGF levels in Type 1 diabetics, (r = 0.76, p < 0.05; 20 min after dye injection) but with free IGF-I levels in type 2 diabetics (r = 0.65, p < 0.05; 5 min after dye injection). IGFBP-3 correlated negatively with capillary permeability in both diabetes types, whereas IGFBP-1 levels correlated positively in Type 2 patients. In conclusion, capillary permeability is increased in both types of diabetes mellitus. However, VEGF and IGF-I may differentially affect microvascular permeability depending on the diabetes type.

Key words:

Capillary Permeability - Diabetes Mellitus - Microangiopathy - VEGF - IGF-I

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1 F. Brausewetter and P. Jehle contributed equally to the work

Priv. Doz. Dr. med. Peter M. Jehle

Department of Internal Medicine II
University of Ulm

Robert Koch-Strasse 8
89081 Ulm
Germany


Phone: + 49 (731) 5000

Fax: + 49 (731) 5002 44 83

Email: peter.jehle@medizin.uni-ulm.de