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DOI: 10.1055/s-2002-19709
Effect of Age on Thrombosis and Morbidity in the Mouse-Limb Transplantation Model
Publication History
Publication Date:
24 January 2002 (online)
ABSTRACT
Murine models of limb transplantation have been recently described. Because of the technical challenge, non-technical factors that influence the success rate and are easily controlled should be considered. This study investigated the effect of recipient animal age on anastomotic thrombosis, morbidity, and mortality. Twelve allogeneic heterotopic hindlimb transplants were performed using the femoral vessels with end-to-end arterial and end-to-side venous anastomoses. Group 1 (n=8) consisted of 2 to 3-month-old mice weighing 17 to 20 g, and Group 2 (n=7) included 7 to 8-month-old mice weighing 24 to 27 g. In Group 1, 6/8 (75 percent) transplants were successful, while in Group 2, only 1/7 (14 percent) allografts survived (p<0.05). A statistically significant difference in the incidence of vascular compromise of the ipsilateral recipient hindlimb was also noted (p<0.01). The authors conclude that although smaller, juvenile, inbred mice have a higher anastomotic patency rate, with greater collateral vasculature in the hindlimbs, and are therefore more suitable for limb transplantation research.
KEYWORD
Age - composite tissue transplantation - limb transplantation - mouse model
REFERENCES
- 1 Zhang Z, Schlachta C, Zhong R. Improved techniques for kidney transplantation in mice. Microsurgery . 1995; 16 103-109
- 2 Zhong R, Zhang Z, Grant D. Development of a mouse intestinal transplantation model. Microsurgery . 1993; 14 141-145
- 3 Koulack J, McAlister V C, Lee T DG. Development of a mouse aortic transplant model of chronic rejection. Microsurgery . 1995; 16 110-113
- 4 Superina R A, Peugh W N, Wood K J, Morris P J. Assessment of primarily vascularized cardiac allografts in mice. Transplantation . 1986; 42 226-227
- 5 Cooley B C, Daley R. Free flap transplantation in mice. Microsurgery . 1998; 18 320-323
- 6 Zhang F, Shi D Y, Buncke H J. Development of a mouse limb transplantation model. Microsurgery . 1999; 19 209-213
- 7 Tung T HH, Mohanakumar T, Mackinnon S E. Development of a mouse model for heterotopic limb and composite-tissue transplantation. J Reconstr Microsurg . 2001; 17 267-73
- 8 Timmerman W, Gassel H J, Ulrichs K, (eds) et al. Organ Transplantation in Rats and Mice.
New York: Springer-Verlag 1998
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- 9 Braam M J, Cooley B C, Gould J S. Topical heparin enhances patency in a rat model of arterial thrombosis. Ann Plast Surg . 1995; 34 148-153
- 10 Min Z, Jones N F. Limb transplantation in rats: immunosuppression with FK-506. J Hand Surg . 1995; 20A 77-87
- 11 Doi K. Homotransplantation of limbs in rats. Plast Reconstr Surg . 1979; 64 613-621
- 12 Kim S K, Aziz S, Oyer P, Hentz V R. Use of cyclosporine-A in allotransplantation of rat limbs. Ann Plast Surg . 1984; 12 249-255
- 13 Press B HJ, Sibley R K, Shons A R. Limb allotransplantation in the rat: extended survival and return of nerve function with continuous cyclosporine/prednisone immunosuppression. Ann Plast Surg . 1986; 16 313-321
- 14 Guzman-Stein A, Shons A R. Functional recovery in the rat limb transplant model: a preliminary study. Transplant Proc . 1987; 19 1115-1117
- 15 Press B HJ, Sibley R K, Shons A R. Modification of experimental limb allograft rejection with cyclosporine and prednisone: a preliminary report. Transplant Proc . 1983; 15 3057-3062
- 16 Kuroki H, Ikuta Y, Akiyama M. Experimental studies of vascularized allogeneic limb transplantation in the rat using a new immunosuppressive agent FK-506: morphological and immunological analysis. Transplant Proc . 1989; 21 3187-3190
- 17 Larsen C P, Pearson T C. The CD40 pathway in allograft rejection, acceptance, and tolerance. Curr Opin Immunol . 1997; 9 641-647
- 18 Denton M D, Reul R M, Dharnidharka V R. Central role for CD40/CD40 ligand (CD154) interactions in transplant rejection. Pediatric Transplantation . 1998; 2 6-15
- 19 Lee W PA, Yaremchuk M J, Pan Y C. Relative antigenicity of components of a vascularized limb allograft. Plast Reconstr Surg . 1991; 87 401-411
- 20 Buttemeyer R, Jones N F, Min, Rao U. Rejection of the component tissues of limb allografts in rats immunosuppressed with FK-605 and cyclosporine. Plast Reconstr Surg . 1996; 97 139-151
- 21 Suzuki H, Hewitt C W, Tran H S. Composite tissue/vascularized bone marrow transplantation: a hierarchy of tissue tolerogenicity. Graft . 1999; 2 111-115
- 22 Lee W PA. Composite tissue transplantation: more science and patience needed. Plast Reconstr Surg . 2001; 107 1066-1070
- 23 Kann B R, Hewitt C W. Composite tissue (hand) allotransplantation: are we ready?. Plast Reconstr Surg . 2001; 107 1060-1065
- 24 Foucher G. Prospects for hand transplantation. Lancet . 1999; 353 1286
- 25 Jensen J N, Mackinnon S E. Composite tissue allotransplantation: a comprehensive review of the literature. Part I. J Reconstr Microsurg . 2000; 16 57-68
- 26 Chick L R, Walton R L, Reus W. Free flaps in the elderly. Plast Reconstr Surg . 1992; 90 87-94
- 27 Shestak K C, Jones N F. Microsurgical free-tissue transfer in the elderly patient. Plast Reconstr Surg . 1991; 88 259-263