Tissue Engineering with Chondrocytes

R. James Koch; Goutham Krishna Gorti

doi:10.1055/s-2002-19828

Facial Plastic Surgery, Table of Contents

Facial Plast Surg 2002; 18(1): 059-068
DOI: 10.1055/s-2002-19828

Tissue Engineering with Chondrocytes

R. James Koch, Goutham Krishna Gorti

Division of Otolaryngology-Head and Neck Surgery, Stanford University Medical Center, Stanford, CA

Abstract

ABSTRACT

Tissue engineering of cartilage, using chondrocytes based on the use of synthetic biodegradable polymer cell delivery vehicles (scaffolds), is an alternate treatment modality for replacing missing cartilage.[1] [2] Cartilage tissue engineering has an important role to play in the generation of graft material for head and neck reconstruction. It is an approach to fabricate cartilage constructs in vitro, which could be used in reconstructive surgery. Methods involve (1) harvesting septal cartilage during septoplasty, (2) isolating chondrocytes through enzymatic digestion of the septal cartilage, (3) expanding the cell number in a two-dimensional monolayer culture, using serum-free media, (4) seeding the cells onto a biodegradable polymer scaffold, and (5) cultivating the seeded scaffolds in a rotating bioreactor. In this article we briefly outline the methodology and clinical applications of cartilage grown ex vivo.

KEYWORD

Tissue engineering - septal cartilage - bioreactor

Full Text

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