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Planta Med 2002; 68(1): 94-96
DOI: 10.1055/s-2002-20051
Letter

© Georg Thieme Verlag Stuttgart · New York

Genetic Identification of Cinnamon (Cinnamomum spp.) Based on the trnL-trnF Chloroplast DNA

Mareshige Kojoma1, 3 , Kogo Kurihara1 , Kazuya Yamada1 , Setsuko Sekita2 , Motoyoshi Satake2 , Osamu Iida1
  • 11 Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Shizuoka, Japan
  • 22 Division of Pharmacognosy and Phytochemistry, National Institute of Health Sciences, Tokyo, Japan
  • 33 Institute of Physical and Chemical Research (RIKEN), Saitama, Japan
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Publication History

February 8, 2001

May 24, 2001

Publication Date:
31 January 2002 (online)

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Abstract

Genetic identification among cinnamon species was studied by analyzing nucleotide sequences of chloroplast DNA from four species (Cinnamomum cassia, C. zeylanicum, C. burmannii and C. sieboldii). The two regions studied were the intergenic spacer region between the trnL 3’exon and trnF exon (trnL -trnF IGS) and the trnL intron region. We found nucleotide variation at one site in the trnL-trnF IGS, and at three sites in the trnL intron. With the sequence data from analysis of these regions, the four Cinnamomum species used in this study were correctly identified. Furthermore, single-strand conformation polymorphism (SSCP) analysis of PCR products from the trnL-trnF IGS and the trnL intron resulted in different SSCP band patterns among C. cassia, C. zeylanicum and C. burmannii.

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Mareshige Kojoma

Izu Experimental Station for Medical Plants

National Institute of Health Sciences

Shizuoka

Japan

Fax: +81-484-62-4674

Email: kojoma@postman.riken.go.jp