Monoamine Oxidase Inhibitory Coumarin from Zanthoxylum schinifolium

Abstract

A methanol extract of Zanthoxylum schinifolium stems at a concentration of 250 μg/ml showed potent inhibitory activity against monoamine oxidase (MAO) in a mouse brain. Activity-guided separation and purification of the extract yielded lacinartin (1) as an active coumarin compound. Lacinartin showed significant inhibitory effects on MAO in a dose-dependent manner. The IC₅₀ value on MAO activity was 9.2μM. The MAO-A (IC₅₀ value, 5.7 μM) sensitivity to lacinartin was greater than that of MAO-B (IC₅₀ value, 28.6μM). An enzyme kinetic study revealed that lacinartin inhibited MAO activity by a non-competitive mode.

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