Planta Med 2002; 68(2): 191-192
DOI: 10.1055/s-2002-20239
Letter

© Georg Thieme Verlag Stuttgart · New York

Authentication of Stems of Dendrobium officinale by rDNA ITS Region Sequences

Xiaoyu Ding1, 2 , Luosan Xu1 , Zhengtao Wang1 , Kaiya Zhou2 , Hong Xu1 , Yiquan Wang2
  • 1Department of Pharmacognosy, China Pharmaceutical University, Nanjing, China
  • 2College of Life Sciences, Nanjing Normal University, Nanjing, China
Further Information

Publication History

March 9, 2001

April 22, 2001

Publication Date:
22 February 2002 (online)

Abstract

The rDNA ITS regions of five Dendrobium species were sequenced. Each Dendrobium species was found to have a unique sequence in the ITS region, so that they could be easily distinguished at the DNA level. The aligned 644 bp of the ITS region includes 235 bp ITS1, 163 bp 5.8S, and 246 bp ITS2. One hundred and eighty-nine sites are variable. The sequences of D. officinale could be easily distinguished from the other four adulterant species according to the sequence variation at 11 sites, 7 in ITS1, 1 in 5.8S, and 3 in ITS2. These could be used as molecular characters to distinguish the stems of D. officinale from the adulterants.

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Prof. Zhou Kaiya

Jiangsu Key Laboratory of Resources Biotechnology

Nanjing Normal University

Nanjing 210097

China

Email: kyzhounj@jlonline.com

Fax: +86-25-359-8328