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DOI: 10.1055/s-2002-20241
© Georg Thieme Verlag Stuttgart · New York
Determination of Ginsenosides from Panax ginseng using Micellar Electrokinetic Chromatography
Publikationsverlauf
February 2, 2001
April 28, 2001
Publikationsdatum:
22. Februar 2002 (online)
![](https://www.thieme-connect.de/media/plantamedica/200202/lookinside/thumbnails/10.1055-s-2002-20241-1.jpg)
Abstract
A method for determination of ginsenosides in herbal medicinal products (HMPs) was developed using micellar electrokinetic chromatography (MEKC). Within 22 minutes 7 major ginsenosides were well separated. In order to demonstrate the accuracy, precision and robustness for the main target analyte the method was exemplarily validated for the determination of Rb1 according to ICH guidelines. Compared to chromatographic analysis, several benefits of capillary electrophoresis (CE) could be demonstrated such as high separation efficiency in an aqueous buffer without any organic solvent and shorter run time per assay.
Key words
Capillary electrophoresis - micellar electrokinetic chromatography - Panax ginseng - Araliaceae - ginsenosides - validation
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Prof. Dr. G. Blaschke
Institute of Pharmaceutical Chemistry
University of Münster
Hittorfstr. 58-62
48149 Münster
Germany
eMail: blaschg@uni-muenster.de
Fax: +49-251-83-32144