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Plant Biol (Stuttg) 2002; 4(1): 53-61
DOI: 10.1055/s-2002-20436
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Nutrients Induce an Increase in Inositol 1,4,5-Trisphosphate in Soybean Cells: Implication for the Involvement of Phosphoinositide-Specific Phospholipase C in DNA Synthesis

T. Shigaki 1, 2 , M. K. Bhattacharyya 1, 3
  • 1 Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73401, USA
  • 2 New address: Plant Physiology Laboratories, Children's Nutrition Research Center, Baylor College of Medicine, 1100 Bates St., Houston, Texas, USA
  • 3 New address: G303 Agronomy Hall, Department of Agronomy, Iowa State University, Ames, Iowa, USA
Further Information

Publication History

August 7, 2001

December 18, 2001

Publication Date:
28 February 2002 (online)

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Abstract

Phosphoinositide-specific phospholipase C (PI-PLC) hydrolyzes the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdInsP2) to generate 1,2-diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (InsP3). Both molecules serve as second messengers to carry out various cellular functions in mammals. In the present study, we demonstrate that many organic and inorganic nutrients cause the elevation of InsP3 concentrations in cultured soybean cells. This elevation of InsP3 content is sustained for several hours following treatment with Murashige-Skoog (MS) inorganic nutrients. Phosphate and calcium are the major components in MS salts responsible for the increase in InsP3 levels. DNA synthesis, a measure of cell growth, was significantly suppressed by the PI-PLC-specific inhibitor 1-(6-{[17β-3-methoxyestra-1,3,5(10)-trien-17-yl]amino}hexyl)-1H-pyrrole-2,5-dione (U-73122), whereas its near-identical analogue 1-(6-{[17β-3-methoxyestra-1,3,5(10)-trien-17-yl]amino}hexyl)-2,5-pyrrolidinedione did not cause any suppression. Activation of PI-PLC by MS salts increased DNA synthesis and abolished the suppression of DNA synthesis caused by U-73122. Thus, we conclude that the higher cellular concentration of InsP3 induced by MS treatment is involved in DNA synthesis.

Abbreviations

DAG: 1,2-diacylglycerol

InsP3: inositol 1,4,5-trisphosphate

PtdInsP2: phosphatidylinositol 4,5-bisphosphate

PI-PLC: phosphoinositide-specific phospholipase C

Key words

InsP3 - phospholipase C - signal transduction - soybean

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M. K. Bhattacharyya

G303 Agronomy Hall
Iowa State University

Ames
Iowa 50011-1010
USA

Email: mbhattac@iastate.edu

Section Editor: A. Läuchli