Download PDF
Planta Med 2002; 68(3): 266-268
DOI: 10.1055/s-2002-23126
Letter

© Georg Thieme Verlag Stuttgart · New York

Effect of Mahonia aquifolium Active Compounds on Interleukin-8 Production in the Human Monocytic Cell Line THP-1

Valéria Hajnická1 , Daniela Košt’álová2 , Danka Švecová3 , Ružena Sochorová3 , Norbert Fuchsberger4 , Jaroslav Tóth2
  • 1Institute of Preventive and Clinical Medicine, Bratislava, Slovakia
  • 2Department of Pharmacognosy and Botany, Faculty of Pharmacy Comenius University, Bratislava, Slovakia
  • 3Clinic of Dermatology, Faculty Hospital and Faculty of Medicine Comenius University, Bratislava, Slovakia
  • 4Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia
Further Information

Publication History

March 16, 2001

June 24, 2001

Publication Date:
25 March 2002 (online)

Also available at
    Permissions and Reprints

Abstract

The effect of the crude extract and of two alkaloid fractions prepared from Mahonia aquifolium on interleukin-8 (IL-8) production in the lipopolysaccharide (LPS)-stimulated human monocytic cell line THP-1 was studied. The production of IL-8 by cells stimulated with 20 ng/ml LPS after 48 h treatment with 20 μg/ml crude extract was inhibited by about 30 %. LPS-stimulated cells treated with 0.1 μg/ml bisbenzylisoquinoline alkaloid fraction exhibited a 40 % inhibition of IL-8 production in comparison with control cells. The fraction of protoberberine alkaloids had no significant inhibitory activity. Weak or no inhibition of IL-8 production was found after treatment with 0.5 μg/ml of protoberberine alkaloids berberine and jatrorrhizine and with berbamine from the group of BBI alkaloids. In contrast, the production was inhibited after treatment with BBI alkaloids baluchistine (about 20 %) and aromoline (up to 30 %).

References

  • 1 Gieler U, von der Weth A, Heger M. Mahonia aquifolium - a new type of topical treatment for psoriasis.  Journal of Dermatological Treatment. 1995;  6 31-4
    PubMedSearch in Google Scholar
  • 2 Bos J D, De Rie A. The pathogenesis of psoriasis: immunological facts and speculation.  Immunology Today. 1999;  20 40-6
    CrossrefPubMedSearch in Google Scholar
  • 3 Okubo Y, Koga M. Peripheral blood monocytes in psoriatic patients overproduce cytokines.  Journal of Dermatological Science. 1998;  17 223-32
    CrossrefPubMedSearch in Google Scholar
  • 4 Kemeny L, Kenderessy A S, Olasz E, Michel G, Ruzicka T, Farkas B, Dobozy A. The interleukin-8 receptor: a potential target for antipsoriatic therapy?.  European Journal of Pharmacology. 1994;  258 269-72
    CrossrefPubMedSearch in Google Scholar
  • 5 Teranishi Y, Mizutani H, Murata M, Shimizu M, Matsushima K. Increased spontaneous production of IL-8 in peripheral blood monocytes from the psoriatic patient: relation to focal infection and response to treatments.  Journal of Dermatological Science. 1995;  10 8-15
    CrossrefPubMedSearch in Google Scholar
  • 6 Sticherling M, Sautier W, Schröder J M, Christophers E. Interleukin-8 plays its role at local level in psoriasis vulgaris.  Acta Dermato-Venereologica. 1999;  79 4-8
    PubMedSearch in Google Scholar
  • 7 Müller K, Ziereis K, Gawlik I. The antipsoriatic Mahonia aquifolium and its active constituents. II. Antiproliferative activity against cell growth of human keratinocytes.  Planta Medica. 1995;  61 74-5
    PubMedSearch in Google Scholar
  • 8 Müller K, Ziereis K. The antipsoriatic Mahonia aquifolium and its active constituents. I. Pro- and antioxidant properties and inhibition of 5-lipoxygenase.  Planta Medica. 1994;  60 421-4
    PubMedSearch in Google Scholar
  • 9 Friedland J S, Remick D G, Shattock R, Griffin G E. Secretion of interleukin-8 following phagocytosis of Mycobacterium tuberculosis by human monocyte cell lines.  European Journal of Immunology. 1992;  22 1373-8
    PubMedSearch in Google Scholar
  • 10 Baqui A A, Meiller T F, Falkler W A. Enhanced interleukin-8 production in THP-1 human monocytic cells by lipopolysaccharide from oral microorganisms and granulocyte-macrophage colony-stimulating factor.  Oral Microbiology and Immunology. 1999;  14 275-80
    CrossrefPubMedSearch in Google Scholar
  • 11 Kemény L, Kenderessy A S, Olasz E, Michel G, Ruzicka T, Farkas B, Dobozy A. The interleukin-8 receptor: a potential target for antipsoriatic therapy?.  European Journal of Pharmacology. 1994;  258 269-72
    CrossrefPubMedSearch in Google Scholar
  • 12 Lee G I, Ha J Y, Min K R, Nakagawa H, Tsurufuji S, Chang I M, Kim Y. Inhibitory effects of oriental herbal medicines on IL-8 induction in lipopolysaccharide-activated rat macrophages.  Planta Medica. 1995;  61 26-30
    Thieme ConnectPubMedSearch in Google Scholar
  • 13 Bezáková L, Misˇík V, Máleková L, Sˇvajdlenka E, Kosˇt’álová D. Lipoxygenase inhibition and antioxidant properties of bisbenzylisoquinoline alkaloids isolated from Mahonia aquifolium .  Pharmazie. 1996;  51 758-61
    PubMedSearch in Google Scholar
  • 14 McCutcheon A R, Ellis S M, Hancock R E, Towers G H. Antifungal screening of medicinal plants of British Columbian native peoples.  Journal of Ethnopharmacology. 1994;  44 157-69
    CrossrefPubMedSearch in Google Scholar
  • 15 Gancevici G G. Bioeffects of the plant Mahonia sempervirens .  Archives Roumaines de Pathologie Experimentales et de Microbiologie. 1990;  49 183-90
    PubMedSearch in Google Scholar
  • 16 Augustin M, Andrees U, Grimme H, Schöpf E, Simon J. Effects of Mahonia aquifolium ointment on the expression of adhesion, proliferation, and activation markers in the skin of patients with psoriasis.  Forschende Komplementarmedizin. 1999;  6 19-21
    PubMedSearch in Google Scholar
  • 17 Kosˇt’álová D, Uhrín D, Hrochová V, Tomko J. A new bisbenzylisoquinoline alkaloid from Mahonia aquifolium (Pursh) Nutt.  Collection of Czechoslovak Chemical Communications. 1987;  52 242-46
    PubMedSearch in Google Scholar
  • 18 Slavík J, Bochorˇáková J, Kosˇt’álová D, Hrochová V. Alkaloids from Mahonia aquifolium (Purch) Nutt. II.  Chemical Papers. 1985;  39 537-42
    PubMedSearch in Google Scholar

Assoc. Prof. Dr. Daniela Košt’álová

Department of Pharmacognosy and Botany

Faculty of Pharmacy, Comenius University

Odbojárov 10

SK-832 32 Bratislava 3

Slovak Republic

Fax: +421-2-55572065

Email: kostalova@fpharm.uniba.sk