Abstract
Four macrocyclic trichothecenes, roridin A, roridin E, verrucarin A and verrucarin J, produced by the hypocrealean epibiont of Baccharis coridifolia , were evaluated for their inhibitory activity against the arenavirus Junin (JUNV), the etiological agent of Argentine hemorrhagic fever. The trichothecenes achieved a dose-dependent inhibition of JUNV multiplication at concentrations not affecting cell viability. The 50 % inhibitory concentration (IC50 ) values determined by a virus yield inhibition assay were in the range 1.2 - 4.9 ng/ml. The most active compound was verrucarin J which reduced JUNV yield more than 2 log units and had a similar effect against the arenavirus Tacaribe. The trichothecenes lacked virucidal effects on JUNV virions. From time of addition and removal experiments, it can be concluded that verrucarin J inhibited a late stage in the replicative cycle of JUNV, after 5 h of adsorption.
Key words
Trichothecenes - Junin virus - arenaviruses - antiviral activity -
Baccharis coridifolia
- Asteraceae
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Elsa B. Damonte
Departamento de Química Biológica
Facultad de Ciencias Exactas y Naturales
UBA
Ciudad Universitaria
Pabellón 2
Piso 4
1428 Buenos Aires
Argentina
Phone: +54-11-4576-3334
Fax: +54-11-4576-3342
Email: edamonte@qb.fcen.uba.ar