Complete LC/MS Analysis of a Tinnevelli Senna Pod Extract and Subsequent Isolation and Identification of Two New Benzophenone Glucosides

Abstract

The hydroalcoholic extract of Tinnevelli senna is widely used as a laxative phytomedicine. In order to improve the knowledge of the chemical composition of this extract, LC/MS and LC/MSⁿ studies were performed, allowing the on-line identification of most of the known constituents, i. e., flavonoids, anthraquinones and the typical dianthronic sennosides. However, the identity of four compounds could not be ascertained on-line under the given LC/MS conditions. These substances were isolated and their structures elucidated as kaempferol, the naphthalene derivative tinnevellin 8-glucoside and two new carboxylated benzophenone glucosides.

Key words

Cassia angustifolia - Leguminosae - LC/MS - tinnevellin 8-glucoside - benzophenone glucosides - cassiaphenone A-2-glucoside - cassiaphenone B-2-glucoside

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